Tavaria M, Gabriele T, Anderson R L, Mirault M E, Baker E, Sutherland G, Kola I
Molecular Genetics and Development Laboratory, Monash University, Melbourne, Victoria, Australia.
Genomics. 1995 Sep 1;29(1):266-8. doi: 10.1006/geno.1995.1242.
The heat shock cognate protein HSP73 (or HSC70) is a member of the HSP70 multigene family. This protein has several functions, including binding to nascent polypeptides to facilitate correct folding and the uncoating of clathrin-coated vesicles. Analysis of somatic cell hybrids by two-dimensional protein gel electrophoresis revealed the presence of a 73-kDa protein in two hybrids containing human chromosomes 5, 6, 9, and 11 in common. Using Western blot analysis, we demonstrate that this protein is a member of the HSP70 family and, by Southern blot analysis, that the HSP73 gene is located on human chromosome 11. Fluorescence in situ hybridization further localized HSP73 to the region 11q23.3-q25. This region is involved in a number of genetic rearrangements and is associated with several well-characterized tumours.
热休克同源蛋白HSP73（或HSC70）是HSP70多基因家族的成员。该蛋白具有多种功能，包括与新生多肽结合以促进正确折叠以及网格蛋白包被小泡的脱包被。通过二维蛋白质凝胶电泳对体细胞杂种进行分析，发现在两个共同含有人类5号、6号、9号和11号染色体的杂种中存在一种73 kDa的蛋白质。通过蛋白质免疫印迹分析，我们证明该蛋白是HSP70家族的成员，并且通过Southern印迹分析表明HSP73基因位于人类11号染色体上。荧光原位杂交进一步将HSP73定位到11q23.3 - q25区域。该区域涉及许多基因重排，并与几种特征明确的肿瘤相关。
