Babu P G, Saraswathi N K, Vaidyanathan H, John T J
Department of Virology & Immunology, Christian Medical College Hospital, Vellore.
Indian J Med Res. 1993 Jan;97:1-3.
The sensitivity of testing pooled sera instead of individual sera for antibody against human immunodeficiency virus (HIV) was evaluated using a non-competitive enzyme-linked immunosorbent assay (ELISA). For this purpose, 42 HIV antibody positive sera were titrated and introduced into 42 sets of pools of 2, 4, 8, 16, 32 or 64 sera in such a manner that each pool had one positive sample and the rest, HIV antibody negative sera. When the pools were tested in ELISA, all pools with high titred antibody positive sera were reactive irrespective of pool size, while some of the pools containing medium or low titred sera were non-reactive when pool size exceeded 16. Subsequently the pool size was limited to 16. When 208 previously unscreened samples were tested in 52 pools of 4, 26 pools of 8 or 13 pools of 16 sera, or individually, 6 antibody positive sera were correctly identified. Thus, it was found that the pooling method did not reduce the sensitivity of ELISA test, whereas the cost was reduced to less than half of that of individual testing.
使用非竞争性酶联免疫吸附测定（ELISA）评估检测人免疫缺陷病毒（HIV）抗体时混合血清而非个体血清的检测敏感性。为此，对42份HIV抗体阳性血清进行滴定，并以每组一个阳性样本，其余为HIV抗体阴性血清的方式，将其分别引入42组由2、4、8、16、32或64份血清组成的混合样本中。当对这些混合样本进行ELISA检测时，所有含有高滴度抗体阳性血清的混合样本均呈反应性，与混合样本大小无关，而当混合样本大小超过16时，一些含有中滴度或低滴度血清的混合样本无反应。随后将混合样本大小限制为16。当对208份先前未筛查的样本以每组4份血清分成52组、每组8份血清分成26组、每组16份血清分成13组进行检测，或单独检测时，正确识别出6份抗体阳性血清。因此，发现混合检测方法并未降低ELISA检测的敏感性，而成本降低至个体检测成本的一半以下。
