Mathieu F, Suwandhi I S, Rekhif N, Millière J B, Lefebvre G
Laboratoire de Microbiologie Industrielle et Alimentaire, ENSAIA, Vandoeuvre, France.
J Appl Bacteriol. 1993 Apr;74(4):372-9. doi: 10.1111/j.1365-2672.1993.tb05141.x.
One hundred and sixty-five isolates of Leuconostoc spp. were tested for bacteriocin production. Only one strain, Leuc. mesenteroides ssp. mesenteroides FR 52, isolated from a raw milk, produced a bacteriocin which was named Mesenterocin 52. This bacteriocin inhibited other Leuconostoc strains and several strains of Enterococcus and Listeria spp. No activity was found against lactococci and lactobacilli. The antibacterial spectrum differed from that of previously described Leuconostoc bacteriocins. Mesenterocin 52 was secreted into the medium during the growth phase. It was inactivated with protease treatments. At pH 7.0 it had a relative stability after heating at 100 degrees C (15 min), but it had a greater stability at pH 4.5 than at pH 7.0 after 6 h at 80 degrees C. The apparent molecular mass was estimated to be less than 10 kDa by ultrafiltration. Mesenterocin 52 showed a bactericidal effect on Leuconostoc paramesenteroides DSM 20288.
对165株明串珠菌属菌株进行了细菌素产生检测。仅从生牛奶中分离出的1株肠膜明串珠菌肠膜亚种FR 52产生了一种细菌素，命名为肠膜菌素52。这种细菌素抑制其他明串珠菌菌株以及几种肠球菌和李斯特菌属菌株。未发现对乳球菌和乳杆菌有活性。其抗菌谱与先前描述的明串珠菌细菌素不同。肠膜菌素52在生长阶段分泌到培养基中。经蛋白酶处理后失活。在pH 7.0时，100℃加热15分钟后具有相对稳定性，但在80℃ 6小时后，在pH 4.5时比在pH 7.0时具有更高的稳定性。通过超滤估计其表观分子量小于10 kDa。肠膜菌素52对类肠膜明串珠菌DSM 20288有杀菌作用。
