Etesse-Carsenti H, Entenza J, Mondain V, Durant J, Pradier C, Bernard E, Dellamonica P
Service des Maladies Infectieuses et Tropicales, Hôpital de l'Archet, Nice.
Pathol Biol (Paris). 1991 May;39(5):414-23.
Fibronectin (Fn) is recognized for its ability to mediate Staphylococcus aureus (SA) adhesion. The number of Fn receptors is dependent on the phase of growth, and type of media. Furthermore, it was of interest to examine SA and Staphylococcus epidermidis (SE) adherence to porcine Fn (IBF 10 ng/ml) immobilized on tissue culture plates. The aim of our study was to examine if removal of FN receptors by growing bacteria in an antibiotic containing medium decreased the ability to adhere and of pefloxacin (P) anti-adhesive properties were medium dependent. As SE usually lack Fn binding capabilities, it seemed of interest to investigate if the effects of subinhibitory concentrations of P were modified by medium or Fn. We found: a) For SA: whether Fn coated onto plastic substrata promoted adherence of strains, P's anti-adhesive properties were saved, non medium or phase of growth dependent. b) For SE: adherence properties were decreased if Fn was coated onto plates. P's anti-adhesive properties were not modified and non medium dependent. The number of Fn receptors on clinical isolates of SA correlates with the invasiveness of the isolates. As P reduced the binding of SA to Fn coated plates, this observation may have important implications for antimicrobial prophylaxis.
纤连蛋白（Fn）因其介导金黄色葡萄球菌（SA）黏附的能力而为人所知。Fn受体的数量取决于生长阶段和培养基类型。此外，研究SA和表皮葡萄球菌（SE）对固定在组织培养板上的猪Fn（10 ng/ml IBF）的黏附情况也很有意义。我们研究的目的是检查在含抗生素的培养基中培养细菌是否会通过去除Fn受体而降低其黏附能力，以及培氟沙星（P）的抗黏附特性是否依赖于培养基。由于SE通常缺乏Fn结合能力，因此研究亚抑制浓度的P的作用是否会因培养基或Fn而改变似乎很有意义。我们发现：a）对于SA：无论包被在塑料基质上的Fn是否促进菌株黏附，P的抗黏附特性均得以保留，不依赖于培养基或生长阶段。b）对于SE：如果Fn包被在平板上，其黏附特性会降低。P的抗黏附特性未改变，且不依赖于培养基。SA临床分离株上Fn受体的数量与分离株的侵袭性相关。由于P降低了SA与包被有Fn的平板的结合，这一观察结果可能对抗菌预防具有重要意义。
