Eroshenko Daria, Morozov Ilya, Korobov Vladimir
Institute of Ecology and Genetics of Microorganisms UB RAS, Perm, Russia,
Curr Microbiol. 2015 Jun;70(6):846-53. doi: 10.1007/s00284-015-0796-8. Epub 2015 Mar 7.
The influence of soluble and immobilized plasma, albumin, and fibronectin (Fn) on the adhesion of three Staphylococcus epidermidis strains to polystyrene was investigated. Both soluble and immobilized plasma and albumin cause to 7-fold reduction of the amounts of adhered cells, regardless of the strain used. The soluble Fn exhibited the adhesion for one strain and did not affect the bacterial sorption for remaining strains, whereas on Fn-coated polystyrene two of the three strains showed about 1.5-fold increase in the number of adsorbed bacteria. The plasma- and albumin-coated surfaces became much more hydrophilic as the contact angle changed from 78 ± 2° for control to 18 ± 2° for plasma and 21 ± 3° for albumin. The ligand-receptor specific interactions strains S. epidermidis with Fn-coated surfaces were proved by measuring the adhesion forces between cell surface and Fn-coated AFM tip. The surface roughness measured using AFM after the plasma and proteins immobilization was changed within 10 nm and not correlate with changes in bacterial adhesion.
研究了可溶性和固定化血浆、白蛋白及纤连蛋白（Fn）对三种表皮葡萄球菌菌株黏附聚苯乙烯的影响。可溶性和固定化血浆及白蛋白均使黏附细胞数量减少7倍，无论使用何种菌株。可溶性Fn对一种菌株表现出黏附作用，而对其余菌株的细菌吸附无影响，然而在涂有Fn的聚苯乙烯上，三种菌株中有两种的吸附细菌数量增加了约1.5倍。血浆和白蛋白包被的表面亲水性显著增强，接触角从对照的78±2°变为血浆的18±2°和白蛋白的21±3°。通过测量细胞表面与涂有Fn的原子力显微镜（AFM）探针之间的黏附力，证实了表皮葡萄球菌菌株与涂有Fn的表面之间存在配体 - 受体特异性相互作用。血浆和蛋白质固定后用AFM测量的表面粗糙度在10纳米内变化，且与细菌黏附的变化无关。
