Ozawa K, Honjo I
Am J Surg. 1977 Mar;133(3):307-11. doi: 10.1016/0002-9610(77)90534-7.
A biochemical analysis of mitochondrial metabolism was made on biopsy specimens of fifteen jaundiced patients. In all but three jaundiced patients the phosphorylative activity based on mitochondrial protein was within normal limits or higher. In nine jaundiced patients the concentrations of cytochrome a(+a3), as representative of respiratory enzymes, increased to more than 1.0 x 10(-10) moles/mg protein, as compared with 0.81 of normal mitochondria, and the relative concentrations of cytochromes, flavoproteins and pyridine nucleotides in relation to cytochrome a(+a3) level remained unchanged. In such patients the phosphorylative activity per unit of cytochrome a(+a3) decreased to approximately 50 per cent of controls, whereas it was within normal limits or greater in jaundiced patients with cytochrome a(+a3) less than 1.0 x 10(-10) moles/mg protein. It is suggested that respiratory enzyme concentrations increase to compensate for the inhibited phosphorylative activity of respiratory assemblies and to maintain the energy balance in the liver in jaundiced patients.
对15例黄疸患者的活检标本进行了线粒体代谢的生化分析。除3例黄疸患者外，其余患者基于线粒体蛋白的磷酸化活性均在正常范围内或更高。作为呼吸酶的代表，9例黄疸患者细胞色素a（+a3）的浓度增加至超过1.0×10（-10）摩尔/毫克蛋白，而正常线粒体为0.81，并且细胞色素、黄素蛋白和吡啶核苷酸相对于细胞色素a（+a3）水平的相对浓度保持不变。在此类患者中，每单位细胞色素a（+a3）的磷酸化活性降至对照组的约50%，而在细胞色素a（+a3）低于1.0×10（-10）摩尔/毫克蛋白的黄疸患者中，该活性在正常范围内或更高。提示在黄疸患者中，呼吸酶浓度增加以补偿呼吸组件受抑制的磷酸化活性，并维持肝脏的能量平衡。
