Cox F, Taylor L, Eskew E K, Mattingly S J
Department of Pediatrics, Medical College of Georgia, Augusta 30912.
J Infect Dis. 1993 May;167(5):1118-22. doi: 10.1093/infdis/167.5.1118.
Pregnant Swiss-Webster mice were vaginally inoculated with 10(5) virulent and avirulent serotype III Streptococcus agalactiae and treated 4 days later with topical vaginal inhibitor solutions. Preparations containing lipoteichoic acid (LTA) or glycerophosphate (GP), the repeating linear backbone of LTA, significantly reduced neonatal colonization and bacteremia by the virulent isolate and colonization by the avirulent strain. Similar results were obtained if bacteria were preincubated with LTA or GP at 37 degrees C for 30 min before vaginal inoculation. Human serum albumin (HSA), a known inhibitor of binding of LTA to human fetal epithelial cells, also resulted in reduction in colonization and bacteremia of neonatal mice. However, maternal treatment with a combination of HSA (2%) and GP (1%) completely prevented neonatal colonization and bacteremia without altering the normal aerobic bacterial vaginal flora. These results provide impetus to the development of an alternative means of preventing neonatal group B streptococcal infections in humans without requiring maternal immunization or chemoprophylaxis.
将10⁵ 株毒力型和无毒力型Ⅲ型无乳链球菌经阴道接种于怀孕的瑞士韦伯斯特小鼠，4天后用局部阴道抑制剂溶液进行处理。含有脂磷壁酸（LTA）或甘油磷酸（GP，LTA的重复线性主链）的制剂显著降低了毒力分离株引起的新生儿定植和菌血症以及无毒力菌株引起的定植。如果在阴道接种前将细菌与LTA或GP在37℃预孵育30分钟，也可获得类似结果。人血清白蛋白（HSA）是一种已知的LTA与人胎儿上皮细胞结合的抑制剂，也可导致新生小鼠定植和菌血症减少。然而，用HSA（2%）和GP（1%）联合对母体进行处理可完全预防新生儿定植和菌血症，且不会改变正常的需氧菌阴道菌群。这些结果为开发一种无需母体免疫或化学预防就能预防人类新生儿B族链球菌感染的替代方法提供了动力。
