人类对杜克雷嗜血杆菌脂寡糖和外膜蛋白的体液免疫反应。

Humoral immune response of humans to lipooligosaccharide and outer membrane proteins of Haemophilus ducreyi.

作者信息

Alfa M J, Olson N, Degagne P, Plummer F, Namaara W, Maclean I, Ronald A R

机构信息

Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, Canada.

出版信息

J Infect Dis. 1993 May;167(5):1206-10. doi: 10.1093/infdis/167.5.1206.

DOI:10.1093/infdis/167.5.1206

PMID:8486955

Abstract

The humoral immune response to purified lipooligosaccharide (LOS) and outer membrane proteins (OMP) of Haemophilus ducreyi was evaluated. Sera from chancroid-endemic (Uganda, Kenya) and -nonendemic (Canada) countries were tested by an ELISA. The response to OMPs was cross-reactive with other Haemophilus species, and elevated levels of antibody were detected in patients that did not have chancroid. The LOS component stimulated an H. ducreyi-specific immune response that was detected only in patients with chancroid. The sensitivity of the LOS ELISA was 96% (95% confidence interval, 89.9%-100%) and the specificity was 97% (95% confidence interval, 95.8%-98.2%). Thus, the anti-H. ducreyi LOS immune response is a significant diagnostic and epidemiologic indicator.

摘要

对杜克雷嗜血杆菌纯化脂寡糖（LOS）和外膜蛋白（OMP）的体液免疫反应进行了评估。通过酶联免疫吸附测定（ELISA）检测了来自软下疳流行国家（乌干达、肯尼亚）和非流行国家（加拿大）的血清。对OMPs的反应与其他嗜血杆菌属物种有交叉反应，在没有软下疳的患者中检测到抗体水平升高。LOS成分刺激了仅在软下疳患者中检测到的杜克雷嗜血杆菌特异性免疫反应。LOS ELISA的敏感性为96%（95%置信区间，89.9%-100%），特异性为97%（95%置信区间，95.8%-98.2%）。因此，抗杜克雷嗜血杆菌LOS免疫反应是一个重要的诊断和流行病学指标。