Brown T J, Ballard R C, Ison C A
Department of Medical Microbiology, St. Mary's Hospital Medical School, Paddington, London, U.K.
Microb Pathog. 1995 Jul;19(1):31-8. doi: 10.1006/mpat.1995.0042.
The specificity of the antibody response to Haemophilus ducreyi in sera from patients attending a sexually transmitted disease clinic in South Africa has been studied using immunoblotting. Patients with chancroid were shown to have higher levels of IgG (mean 0.74, SD 0.34) to H. ducreyi than those with no history of chancroid (mean 0.34, SD 0.19). The pattern of the antibody specificity was highly variable between patients with culture proven chancroid but there was no observed strain specificity. In comparison, the patterns obtained using sera from patients without known exposure to H. ducreyi showed less variation between patients and were of less intensity at the dilution used. Sera from patients with chancroid recognised epitopes on proteins that varied in molecular weight between strains, particularly of 60-66kDa (10 of 36 patients) and 25-27kDa (8 of 36 patients). In addition epitopes were recognised on the GroEL and/or DnaK heat shock proteins in 13 of 36 sera tested. There was no apparent change in the epitopes recognised on proteins between the homologous and heterologous strains. Patterns of antibody specificity in sequential sera only varied in one of six patients tested.
利用免疫印迹法，对南非一家性传播疾病诊所患者血清中针对杜克雷嗜血杆菌的抗体反应特异性进行了研究。结果显示，患有软下疳的患者血清中针对杜克雷嗜血杆菌的IgG水平（均值0.74，标准差0.34）高于无软下疳病史的患者（均值0.34，标准差0.19）。在经培养证实患有软下疳的患者中，抗体特异性模式差异很大，但未观察到菌株特异性。相比之下，使用未接触过杜克雷嗜血杆菌患者的血清得到的模式在患者之间差异较小，且在所用稀释度下强度较低。患有软下疳的患者血清识别出不同菌株间分子量不同的蛋白质上的表位，尤其是60 - 66kDa（36名患者中有10名）和25 - 27kDa（36名患者中有8名）的蛋白质。此外，在36份检测血清中的13份中，还识别出了GroEL和/或DnaK热休克蛋白上的表位。同源菌株和异源菌株蛋白质上识别出的表位没有明显变化。在检测的6名患者中，只有1名患者连续血清中的抗体特异性模式有所不同。
