Lange T M, Wood T O, McLaughlin B J
Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Research Institute, University of Louisville School of Medicine 40292.
J Cataract Refract Surg. 1993 Mar;19(2):232-5. doi: 10.1016/s0886-3350(13)80947-9.
Rabbit corneal endothelial cells were transplanted into the right eyes of four New Zealand white rabbits using bovine Descemet's membrane as a cell carrier. Descemet's membranes were dissected from cow eyes, flattened on 36 mm culture dishes, and cut into discs with a 6 mm trephine. Rabbit corneal endothelial cells were seeded onto the discs and maintained in tissue culture conditions for seven days until a complete monolayer with a cell density of 3,000 cells/mm2 was formed. Before transplantation, corneal edema was induced in the host animals by an endothelial scrape wound, which removed the corneal endothelium. Five weeks later, the animals were prepared for transplantation. A corneal button was cut and placed on a dissection table so the host Descemet's membrane could be removed. The donor Descemet's membrane with a complete monolayer of rabbit corneal endothelial cells was placed on the stroma of the corneal button. To keep the donor membrane in place, the button was cauterized at three peripheral points and sutured back into the host eye. Prednisolone sodium phosphate eyedrops and dexamethasone eyedrops and ointment were applied twice daily during the post-operative period. All grafts stayed clear for a period of 12 to 17 weeks. This study shows the potential for using Descemet's membrane as a carrier for corneal endothelial cell transplantation.
将兔角膜内皮细胞以牛Descemet膜作为细胞载体移植到4只新西兰白兔的右眼。从牛眼中取出Descemet膜，展平在36毫米培养皿上，用6毫米环钻切成圆盘状。将兔角膜内皮细胞接种到圆盘上，并在组织培养条件下培养7天，直至形成细胞密度为3000个细胞/平方毫米的完整单层。移植前，通过内皮刮伤在宿主动物中诱导角膜水肿，刮伤去除角膜内皮。5周后，对动物进行移植准备。切下一个角膜片并放置在解剖台上，以便去除宿主的Descemet膜。将带有完整单层兔角膜内皮细胞的供体Descemet膜放置在角膜片的基质上。为了使供体膜固定在位，在角膜片的三个周边点进行烧灼，并缝合回宿主眼中。术后期间，每天两次应用泼尼松龙磷酸钠滴眼液、地塞米松滴眼液和眼膏。所有移植物在12至17周的时间内保持透明。这项研究表明了使用Descemet膜作为角膜内皮细胞移植载体的潜力。
