Kosugi H, Kojima T, Kikugawa K
Ferris University, Yokohama, Japan.
Lipids. 1993 Apr;28(4):337-43. doi: 10.1007/BF02536320.
A 532 nm red pigment formed in the thiobarbituric acid (TBA) assay of human urine was characterized after separation of the pigment by high-performance liquid chromatography. The yield of the red pigment was somewhat higher at pH 2 than at pH 5; its development was not inhibited by ethylenediaminetetraacetic acid. The characteristics of the pigment were similar to those of the pigment derived from standard malonaldehyde. The amount of the pigment formed was roughly equal to the content of malonaldehyde derivatives estimated as 1-(2,4-dinitrophenyl)pyrazole. Pigment formation was significantly enhanced by t-butyl hydroperoxide (t-BuOOH) and ferric ions, which may be due to pigment formed from aldehydes other than malonaldehyde; the presence of these aldehydes was confirmed by the formation of the corresponding 2,4-dinitrophenylhydrazones. The amount of pigment produced from 24-h urine samples of 12 healthy subjects was estimated to be 26-95 nmol/kg, and 65-182 nmol/kg in the presence of t-BuOOH. These values are lower than those for urine of rabbit or rat. The TBA reactivity in the absence and presence of t-BuOOH of human urine was not related to age or sex. The TBA reactivity of human urine collected in the afternoon and in the evening was higher than that of urine collected in the morning.
在通过高效液相色谱法分离人尿硫代巴比妥酸（TBA）测定中形成的532nm红色色素后，对其进行了表征。红色色素在pH 2时的产量略高于pH 5时；其形成不受乙二胺四乙酸的抑制。该色素的特性与源自标准丙二醛的色素相似。形成的色素量大致等于以1-（2,4-二硝基苯基）吡唑估算的丙二醛衍生物的含量。叔丁基过氧化氢（t-BuOOH）和铁离子可显著增强色素形成，这可能是由于除丙二醛之外的醛形成了色素；通过相应的2,4-二硝基苯腙的形成证实了这些醛的存在。12名健康受试者24小时尿液样本产生的色素量估计为26 - 95 nmol/kg，在存在t-BuOOH的情况下为65 - 182 nmol/kg。这些值低于兔或大鼠尿液的值。人尿在不存在和存在t-BuOOH时的TBA反应性与年龄或性别无关。下午和晚上收集的人尿的TBA反应性高于早上收集的尿液。
