Bird R P, Hung S S, Hadley M, Draper H H
Anal Biochem. 1983 Jan;128(1):240-4. doi: 10.1016/0003-2697(83)90371-8.
An HPLC method is described for the determination of malonaldehyde in biological materials. The procedure involves extracting the sample with trichloracetic acid, heating the extract with thiobarbituric acid, separating the thiobarbituric acid-malonaldehyde complex on a mu Bondapak C18 column, and measuring the absorbance using a 546-nm interference filter. The method was found to be specific for malonaldehyde in several food and feed samples. Under routine assay conditions, a coefficient of variability of 7.0% was obtained for samples containing 1-2 microgram of malonaldehyde per gram (instrument detection limit 1 ng). This procedure yields lower values for the concentration of malonaldehyde in food samples than the conventional spectrophotometric procedure based on absorbance of the thiobarbituric acid-malonaldehyde complex at 532 nm.
描述了一种用于测定生物材料中丙二醛的高效液相色谱法。该程序包括用三氯乙酸萃取样品，将提取物与硫代巴比妥酸加热，在μ Bondapak C18柱上分离硫代巴比妥酸 - 丙二醛复合物，并使用546 nm干涉滤光片测量吸光度。发现该方法对几种食品和饲料样品中的丙二醛具有特异性。在常规测定条件下，每克含有1 - 2微克丙二醛的样品（仪器检测限为1 ng）的变异系数为7.0%。与基于硫代巴比妥酸 - 丙二醛复合物在532 nm处吸光度的传统分光光度法相比，该程序得出的食品样品中丙二醛浓度值更低。
