Thompson J, Mössinger S, Reichardt V, Engels U, Beauchemin N, Kommoss F, von Kleist S, Zimmermann W
Institute of Immunobiology, Freiburg University, Germany.
Int J Cancer. 1993 Sep 9;55(2):311-9. doi: 10.1002/ijc.2910550223.
Carcinoembryonic antigen (CEA) is a tumor marker that belongs to a family of closely related molecules with variable expression patterns. We have developed sets of oligonucleotide primers for the specific amplification of transcripts from individual CEA-family members using the reverse transcriptase/polymerase chain reaction (RT/PCR). Specific primer sets were designed for CEA, non-specific cross-reacting antigen (NCA), biliary glycoprotein (BGP), carcinoembryonic antigen gene-family members 1, 6 and 7 (CGM1, CGM6 and CGM7), and one set for all pregnancy-specific glycoprotein (PSG) transcripts. Primers were first tested for their specificity against individual cDNA clones and product-hybridization with internal, transcript-specific oligonucleotides. Total RNA from 12 brain and 63 gynecological tumors were then tested for expression of CEA-related transcripts. None were found in tumors located in the brain, including various mesenchymal and neuro-epithelial tumors. CEA and NCA transcripts were, however, present in an adenocarcinoma located in the nasal sinuses. In ovarian mucinous adenocarcinomas, we always found co-expression of CEA and NCA transcripts, and occasionally BGP mRNA. CEA-related transcripts were also found in some serous, endometrioid and clear-cell ovarian carcinomas. CEA, NCA and BGP transcripts were present in endometrial carcinomas of the uterus and cervical carcinomas, whereas uterine leiomyomas were completely negative. No transcripts were found from CGM1, CGM6, CGM7 or from PSG genes in any of the tumors tested. The PCR data were compared with immunohistochemical investigations of ovarian tumors at the protein level using CEA (26/3/13)-, NCA-50/90 (9A6FR) and NCA-95 (80H3)-specific monoclonal antibodies.
癌胚抗原（CEA）是一种肿瘤标志物，属于一组密切相关且表达模式各异的分子家族。我们利用逆转录酶/聚合酶链反应（RT/PCR）开发了多组寡核苷酸引物，用于特异性扩增各个CEA家族成员的转录本。针对CEA、非特异性交叉反应抗原（NCA）、胆汁糖蛋白（BGP）、癌胚抗原基因家族成员1、6和7（CGM1、CGM6和CGM7）设计了特异性引物组，还设计了一组用于扩增所有妊娠特异性糖蛋白（PSG）转录本的引物。首先针对各个cDNA克隆测试引物的特异性，并测试产物与内部转录本特异性寡核苷酸的杂交情况。然后检测了来自12例脑肿瘤和63例妇科肿瘤的总RNA中CEA相关转录本的表达情况。在包括各种间叶组织和神经上皮肿瘤在内的脑肿瘤中均未发现相关转录本。然而，鼻窦腺癌中存在CEA和NCA转录本。在卵巢黏液性腺癌中，我们总是发现CEA和NCA转录本共表达，偶尔也有BGP mRNA表达。在一些浆液性、子宫内膜样和透明细胞卵巢癌中也发现了CEA相关转录本。子宫子宫内膜癌和宫颈癌中存在CEA、NCA和BGP转录本，而子宫平滑肌瘤则完全呈阴性。在所检测的任何肿瘤中均未发现CGM1、CGM6、CGM7或PSG基因的转录本。将PCR数据与使用CEA（26/3/13）、NCA-50/90（9A6FR）和NCA-95（80H3）特异性单克隆抗体在蛋白质水平对卵巢肿瘤进行的免疫组织化学研究结果进行了比较。
