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[凝胶微试验在输血前血清学检测中用于相容性检测的应用]

[Use of microtest in gel for compatibility in serological testing before transfusion].

作者信息

Kuśnierz-Alejska G, Fabijańska-Mitek J

机构信息

Zakładu Serologii Instytutu Hematologii i Transfuzjologii, Warszawie.

出版信息

Acta Haematol Pol. 1993;24(1):49-56.

PMID:8488736
Abstract

A gel technique for the detection of red blood cell antigen--antibody reaction was evaluated in pretransfusion compatibility tests. The evaluation was undertaken on 917 blood samples of recipients and 2450 samples of donors. Parallel study of microtest in gel and classical techniques revealed that gel technique was more sensitive than the classical one, because some antibodies of Rh, Kell and Kidd systems were found by this method exclusively. It is also more specific because a lot of unspecific reactions could be eliminated. Therefore, it was decided to use the microtest in gel for crossmatching of recipient serum against red cells from each intended donor--particularly when the patient had irregular erythrocyte antibodies. The results presented in this paper revealed that in gel is one of the most potent tests and it is the safest for routine use before transfusion.

摘要

一种用于检测红细胞抗原 - 抗体反应的凝胶技术在输血前相容性试验中进行了评估。对917份受血者血样和2450份供血者血样进行了评估。凝胶微试验与传统技术的平行研究表明,凝胶技术比传统技术更敏感,因为通过这种方法专门发现了Rh、Kell和Kidd系统的一些抗体。它也更具特异性,因为可以消除许多非特异性反应。因此,决定使用凝胶微试验对受血者血清与每个预期供血者的红细胞进行交叉配血——特别是当患者有不规则红细胞抗体时。本文给出的结果表明,凝胶试验是最有效的试验之一,也是输血前常规使用最安全的试验。

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