磷脂双层与猪心AMP脱氨酶的相互作用：傅里叶变换红外光谱和动力学研究。
The interaction of phospholipid bilayers with pig heart AMP deaminase: Fourier-transform infrared spectroscopic and kinetic studies.
作者信息
Tanfani F, Kossowska E, Purzycka-Preis J, Zydowo M M, Wozniak M, Tartaglini E, Bertoli E
机构信息
Istituto di Biochimica, Facoltà di Medicina, Università di Ancona, Italy.
出版信息
Biochem J. 1993 May 1;291 ( Pt 3)(Pt 3):921-6. doi: 10.1042/bj2910921.
The interaction of pig heart AMP deaminase with different chemical species of phosphatidylcholine and with natural plasma membranes has been investigated. Phospholipids added to the system either as natural biological membranes (plasma membrane vesicles) or in the form of liposomes containing unsaturated phosphatidylcholine considerably enhanced AMP deaminase activity. The secondary structure of pig heart AMP deaminase in the absence and in the presence of dioleoyl phosphatidylcholine and dipalmitoyl phosphatidylcholine liposomes was investigated by Fourier-transform infrared spectroscopy. Quantitative analysis of the amide I band showed that the enzyme contains 45% beta-sheets, 28% alpha-helix, 16% turns and 11% non-ordered structure. In the presence of dioleoyl phosphatidylcholine liposomes, the beta/alpha content ratio decreased; this decrease was dependent on the amount of lipid added. This phenomenon was not observed in the case of dipalmitoyl phosphatidylcholine liposomes. These data suggest a possible role for membrane phospholipids in the regulation of AMP deaminase activity.
研究了猪心AMP脱氨酶与不同化学种类的磷脂酰胆碱以及天然质膜之间的相互作用。作为天然生物膜（质膜囊泡）或含有不饱和磷脂酰胆碱的脂质体形式添加到系统中的磷脂，可显著增强AMP脱氨酶的活性。通过傅里叶变换红外光谱研究了在不存在和存在二油酰磷脂酰胆碱和二棕榈酰磷脂酰胆碱脂质体的情况下猪心AMP脱氨酶的二级结构。酰胺I带的定量分析表明，该酶含有45%的β-折叠、28%的α-螺旋、16%的转角和11%的无序结构。在存在二油酰磷脂酰胆碱脂质体的情况下，β/α含量比降低；这种降低取决于添加的脂质数量。在二棕榈酰磷脂酰胆碱脂质体的情况下未观察到这种现象。这些数据表明膜磷脂在调节AMP脱氨酶活性方面可能发挥作用。
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