Whyte B J, Griffiths W T
Department of Biochemistry, School of Medical Sciences, University of Bristol, U.K.
Biochem J. 1993 May 1;291 ( Pt 3)(Pt 3):939-44. doi: 10.1042/bj2910939.
A technique involving solid-phase extractions and polyethylene h.p.l.c. suitable for the routine compositional analysis of the total protochlorophyllide pool of plants is described. The resynthesis kinetics of the individual components of the pool have been studied in briefly illuminated etiolated tissue of wheat (Triticum aestivum) and cucumber (Cucumis sativus) during subsequent redarkening. The data are interpreted in terms of a precursor-product relationship between the di- and mono-vinyl analogues of protochlorophyllide during their reaccumulation in darkness. The interconversion is assumed to be catalysed by an 8-vinyl reductase, which shows greater activity in wheat than in cucumber. Analyses of the redox state of the nicotinamide nucleotide of the pool during the process are compatible with NADPH as the cofactor of the putative reductase.
本文描述了一种涉及固相萃取和聚乙烯高效液相色谱的技术,该技术适用于植物原叶绿素酸酯总库的常规成分分析。在随后的再黑暗处理过程中,对小麦(Triticum aestivum)和黄瓜(Cucumis sativus)黄化组织短暂光照后,研究了该库中各个组分的再合成动力学。根据原叶绿素酸酯的二乙烯基和单乙烯基类似物在黑暗中重新积累时的前体-产物关系对数据进行了解释。假定这种相互转化由一种8-乙烯基还原酶催化,该酶在小麦中的活性高于黄瓜。在此过程中对该库中烟酰胺核苷酸氧化还原状态的分析与NADPH作为假定还原酶的辅因子相一致。