Hanamoto C M, Castelfranco P A
Department of Botany, University of California, Davis, California 95616.
Plant Physiol. 1983 Sep;73(1):79-81. doi: 10.1104/pp.73.1.79.
A method was developed to separate the monovinyl and divinyl forms of protochlorophyllide and chlorophyllide by high pressure liquid chromatography using a silicic acid column coated with dodecyl residues and a moving phase containing the lipophilic cation, tetrabutyl ammonium. The solvent was 70% methyl alcohol containing varying amounts of methyl ethyl ketone. The separation was carried out at 0 degrees C. This method was used to test and confirm a previous report that, in cucumber cotyledons, divinyl protochlorophyllide is phototransformed to give divinyl chlorophyllide, which is biologically unstable and disappears rapidly in the dark.
已开发出一种方法，通过高压液相色谱法分离原叶绿素酸酯和叶绿素酸酯的单乙烯基和二乙烯基形式，该方法使用涂有十二烷基残基的硅酸柱和含有亲脂性阳离子四丁基铵的流动相。溶剂为含有不同量甲乙酮的70%甲醇。分离在0℃下进行。该方法用于检验和证实先前的一份报告，即在黄瓜子叶中，二乙烯基原叶绿素酸酯经光转化生成二乙烯基叶绿素酸酯，后者在生物学上不稳定，在黑暗中会迅速消失。
