Belanger F C, Duggan J X, Rebeiz C A
J Biol Chem. 1982 May 10;257(9):4849-58.
The chemical identification of chlorophyllide (E458F674) (Belanger, F. C., and Rebeiz, C. A. (1980) Plant Sci. Lett. 18, 343-350) has been confirmed by chemical derivatization coupled to spectrofluorometric, spectrophotometric, and chromatographic analysis. Chlorophyllide (E458F674) and its demetallated analog were converted by catalytic hydrogenation into mesochlorophyllide a and mesopheophorbide a. Furthermore, methyl chlorophyllide (E458F674) was converted by partial hydrogenation into a mixture of monovinyl chlorophyllide a isomers and the latter into mesochlorophyllide a by further hydrogenation. On the other hand, chemical oxidation of methyl chlorophyllide (E458F674) converted it into methyl divinyl protochlorophyllide. Chlorophyllide (E458F674) was detected in several plant species and is proposed to be an important intermediate of the chlorophyll a biosynthetic pathway.
通过化学衍生化结合荧光光谱、分光光度和色谱分析，已证实叶绿素酸酯（E458F674）（贝朗热，F.C.，和雷贝兹，C.A.（1980年）《植物科学快报》18卷，343 - 350页）的化学鉴定结果。叶绿素酸酯（E458F674）及其脱金属类似物通过催化氢化转化为中叶绿素酸酯a和中脱镁叶绿素酸酯a。此外，甲基叶绿素酸酯（E458F674）通过部分氢化转化为单乙烯基叶绿素酸酯a异构体的混合物，后者通过进一步氢化转化为中叶绿素酸酯a。另一方面，甲基叶绿素酸酯（E458F674）的化学氧化将其转化为甲基二乙烯基原叶绿素酸酯。在几种植物物种中检测到了叶绿素酸酯（E458F674），并提出它是叶绿素a生物合成途径的重要中间体。
