对七种用于单核细胞增生李斯特菌流行病学分型的国际噬菌体集的实验性噬菌体进行评估。
Evaluation of seven experimental phages for inclusion in the international phage set for the epidemiological typing of Listeria monocytogenes.
作者信息
van der Mee-Marquet N, Loessner M, Audurier A
机构信息
Laboratoire de Microbiologie, Faculté de Médecine, Tours, France.
出版信息
Appl Environ Microbiol. 1997 Sep;63(9):3374-7. doi: 10.1128/aem.63.9.3374-3377.1997.
The purpose of our study was to evaluate the inclusion of seven experimental phages into the international phage set for subtyping Listeria monocytogenes. The seven additional phages included the broad-host-range virulent Myoviridae phage A511 (M. J. Loessner, Appl. Environ. Microbiol. 57:1912-1918, 1991), three temperate phages from the Danish subsystem for typing serotype 1/2 strains (12682, 6223, and 5775) (P. Gerner-Smidt, V.T. Rosdahl, and W. Frederiksen, APMIS 101:160-167, 1993), and three temperate phages isolated by this laboratory in France (9425, 1313, and 197). A panel of 395 Listeria monocytogenes isolates (including 180 that were non-phage typeable by the international set) were used in the study for a comparison of the lytic spectra of the various bacteriophages. These results showed that the inclusion of five of the experimental phages contributed greatly to the overall typeability and discriminatory power of the system, especially for strains within serogroup 1/2.
我们研究的目的是评估将七种实验性噬菌体纳入用于单核细胞增生李斯特菌分型的国际噬菌体组合的情况。这七种额外的噬菌体包括广宿主范围的烈性肌尾噬菌体科噬菌体A511（M. J. 洛斯纳，《应用与环境微生物学》57:1912 - 1918，1991年）、来自丹麦血清型1/2菌株分型子系统的三种温和噬菌体（12682、6223和5775）（P. 格纳 - 施密特、V. T. 罗斯达尔和W. 弗雷德里克森，《APMIS》101:160 - 167，1993年），以及本实验室在法国分离的三种温和噬菌体（9425、1313和197）。一组395株单核细胞增生李斯特菌分离株（包括180株不能被国际组合进行噬菌体分型的菌株）用于该研究，以比较各种噬菌体的裂解谱。这些结果表明，纳入五种实验性噬菌体对该系统的总体分型能力和鉴别力有很大贡献，尤其是对于血清群1/2内的菌株。
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