基于聚合酶链式反应（PCR）的对石韦（水龙骨科石韦属植物石韦）和拟南芥中编码肌球蛋白样蛋白的部分互补DNA（cDNA）的扩增及序列特征分析
PCR-dependent amplification and sequence characterization of partial cDNAs encoding myosin-like proteins in Anemia phyllitidis (L.) Sw. and Arabidopsis thaliana (L.) Heynh.
作者信息
Moepps B, Conrad S, Schraudolf H
机构信息
Institut für Allgemeine Botanik, Universität Ulm, FRG.
出版信息
Plant Mol Biol. 1993 Mar;21(6):1077-83. doi: 10.1007/BF00023604.
PMID:8490128
Abstract
Partial cDNAs encoding for myosin-like proteins from Anemia phyllitidis and Arabidopsis thaliana have been isolated using PCR technology. The deduced amino acid sequences show an average similarity up to 62% with known myosin heavy chain genes. From northern blot analysis we were able to estimate that transcripts of ca. 6.1 kb size are expressed in A. phyllitidis.
摘要
利用聚合酶链反应（PCR）技术，已从扇叶铁线蕨（Anemia phyllitidis）和拟南芥（Arabidopsis thaliana）中分离出编码类肌球蛋白蛋白的部分互补DNA（cDNA）。推导的氨基酸序列与已知的肌球蛋白重链基因平均相似度高达62%。通过Northern印迹分析，我们能够估计在扇叶铁线蕨中表达的转录本大小约为6.1 kb。
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