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基于聚合酶链式反应（PCR）的对石韦（水龙骨科石韦属植物石韦）和拟南芥中编码肌球蛋白样蛋白的部分互补DNA（cDNA）的扩增及序列特征分析

PCR-dependent amplification and sequence characterization of partial cDNAs encoding myosin-like proteins in Anemia phyllitidis (L.) Sw. and Arabidopsis thaliana (L.) Heynh.

作者信息

Moepps B, Conrad S, Schraudolf H

机构信息

Institut für Allgemeine Botanik, Universität Ulm, FRG.

出版信息

Plant Mol Biol. 1993 Mar;21(6):1077-83. doi: 10.1007/BF00023604.

DOI:10.1007/BF00023604
PMID:8490128
Abstract

Partial cDNAs encoding for myosin-like proteins from Anemia phyllitidis and Arabidopsis thaliana have been isolated using PCR technology. The deduced amino acid sequences show an average similarity up to 62% with known myosin heavy chain genes. From northern blot analysis we were able to estimate that transcripts of ca. 6.1 kb size are expressed in A. phyllitidis.

摘要

利用聚合酶链反应（PCR）技术，已从扇叶铁线蕨（Anemia phyllitidis）和拟南芥（Arabidopsis thaliana）中分离出编码类肌球蛋白蛋白的部分互补DNA（cDNA）。推导的氨基酸序列与已知的肌球蛋白重链基因平均相似度高达62%。通过Northern印迹分析，我们能够估计在扇叶铁线蕨中表达的转录本大小约为6.1 kb。

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本文引用的文献

1
Identification of common molecular subsequences.常见分子子序列的鉴定
J Mol Biol. 1981 Mar 25;147(1):195-7. doi: 10.1016/0022-2836(81)90087-5.
2
Genomic sequencing.基因组测序
Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5. doi: 10.1073/pnas.81.7.1991.
3
pEMBL: a new family of single stranded plasmids.pEMBL：一类新型单链质粒。
盘基网柄菌肌球蛋白J：广义定义的肌球蛋白V类成员还是XI类非常规肌球蛋白？
J Muscle Res Cell Motil. 1996 Aug;17(4):411-24. doi: 10.1007/BF00123358.
4
Molecular analysis of the myosin gene family in Arabidopsis thaliana.拟南芥肌球蛋白基因家族的分子分析
Plant Mol Biol. 1994 Nov;26(4):1139-53. doi: 10.1007/BF00040695.
Nucleic Acids Res. 1983 Mar 25;11(6):1645-55. doi: 10.1093/nar/11.6.1645.
4
An alternative approach to deoxyoligonucleotides as hybridization probes by insertion of deoxyinosine at ambiguous codon positions.通过在模糊密码子位置插入脱氧肌苷，将脱氧寡核苷酸用作杂交探针的另一种方法。
J Biol Chem. 1985 Mar 10;260(5):2605-8.
5
Anti-tubulin antibodies locate the blepharoplast during spermatogenesis in the fern Platyzoma microphyllum R.Br.: a correlated immunofluorescence and electron-microscopic study.
J Cell Sci. 1986 Mar;81:243-65. doi: 10.1242/jcs.81.1.243.
6
Myosin structure and function in cell motility.肌球蛋白在细胞运动中的结构与功能。
Annu Rev Cell Biol. 1987;3:379-421. doi: 10.1146/annurev.cb.03.110187.002115.
7
Myosins of nonmuscle cells.非肌肉细胞的肌球蛋白
Annu Rev Biophys Biophys Chem. 1988;17:23-45. doi: 10.1146/annurev.bb.17.060188.000323.
8
Progressive sequence alignment as a prerequisite to correct phylogenetic trees.渐进序列比对是构建正确系统发育树的前提条件。
J Mol Evol. 1987;25(4):351-60. doi: 10.1007/BF02603120.
9
Complete nucleotide sequence of full length cDNA for rat alpha cardiac myosin heavy chain.大鼠α心肌肌球蛋白重链全长cDNA的完整核苷酸序列
Nucleic Acids Res. 1989 Sep 25;17(18):7527-8. doi: 10.1093/nar/17.18.7527.
10
Partial deduced sequence of the 110-kD-calmodulin complex of the avian intestinal microvillus shows that this mechanoenzyme is a member of the myosin I family.禽类肠道微绒毛110-kD钙调蛋白复合体的部分推导序列表明，这种机械酶是肌球蛋白I家族的成员。
J Cell Biol. 1989 Dec;109(6 Pt 1):2895-903. doi: 10.1083/jcb.109.6.2895.