• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

来自蕨类植物荷叶铁线蕨的编码γ-微管蛋白的cDNA的分离、特性鉴定及序列分析

Isolation, characterization and sequence of a cDNA encoding gamma-tubulin protein from the fern Anemia phyllitidis L. Sw.

作者信息

Fuchs U, Moepps B, Maucher H P, Schraudolf H

机构信息

Abteilung Allgemeine Botanik, Universität Ulm, Germany.

出版信息

Plant Mol Biol. 1993 Nov;23(3):595-603. doi: 10.1007/BF00019306.

DOI:10.1007/BF00019306
PMID:8219092
Abstract

A complete cDNA encoding gamma-tubulin protein from Anemia phyllitidis is presented. The deduced amino acid sequence shows an average similarity of 81-86% to known gamma-tubulin genes from insects, mammals and fungi. Northern blots indicate the existence of transcripts of ca. 1.9 kb which are expressed during gametogenesis of A. phyllitidis. The gene described is the first cloned and entirely sequenced gamma-tubulin gene in any plant.

摘要

本文展示了来自叶状贫血菌(Anemia phyllitidis)的编码γ-微管蛋白的完整cDNA。推导的氨基酸序列与昆虫、哺乳动物和真菌已知的γ-微管蛋白基因平均相似度为81-86%。Northern杂交表明存在约1.9 kb的转录本,其在叶状贫血菌的配子发生过程中表达。所描述的基因是任何植物中第一个克隆并完全测序的γ-微管蛋白基因。

相似文献

1
Isolation, characterization and sequence of a cDNA encoding gamma-tubulin protein from the fern Anemia phyllitidis L. Sw.来自蕨类植物荷叶铁线蕨的编码γ-微管蛋白的cDNA的分离、特性鉴定及序列分析
Plant Mol Biol. 1993 Nov;23(3):595-603. doi: 10.1007/BF00019306.
2
PCR-dependent amplification and sequence characterization of partial cDNAs encoding myosin-like proteins in Anemia phyllitidis (L.) Sw. and Arabidopsis thaliana (L.) Heynh.基于聚合酶链式反应(PCR)的对石韦(水龙骨科石韦属植物石韦)和拟南芥中编码肌球蛋白样蛋白的部分互补DNA(cDNA)的扩增及序列特征分析
Plant Mol Biol. 1993 Mar;21(6):1077-83. doi: 10.1007/BF00023604.
3
Detection and partial sequence of phytochrome genes in the ferns Anemia phyllitidis (L.)Sw (Schizaeaceae) and Dryopteris filix-mas L. (Polypodiaceae) by using polymerase-chain reaction technology.利用聚合酶链反应技术对蕨类植物小叶铁角蕨(铁角蕨科)和鳞毛蕨(鳞毛蕨科)中的光敏色素基因进行检测及部分序列分析
Photochem Photobiol. 1992 Nov;56(5):759-63. doi: 10.1111/j.1751-1097.1992.tb02231.x.
4
Primary structure of Neurospora crassa gamma-tubulin.粗糙脉孢菌γ-微管蛋白的一级结构。
Gene. 1997 Oct 15;199(1-2):303-9. doi: 10.1016/s0378-1119(97)00410-1.
5
Unusual distribution of gamma-tubulin in the giant fresh water amoeba Reticulomyxa filosa.γ-微管蛋白在大型淡水变形虫丝状网柱黏菌中的异常分布。
Eur J Cell Biol. 1997 Apr;72(4):287-96.
6
A highly conserved alpha-tubulin sequence from Prunus amygdalus.来自扁桃的一个高度保守的α-微管蛋白序列。
Plant Mol Biol. 1993 Aug;22(5):913-6. doi: 10.1007/BF00027377.
7
The beta-tubulin-encoding gene from Microbotryum violaceum: unusual in a variety of ways.来自堇菜碎黑粉菌的β-微管蛋白编码基因:在多种方面都很独特。
Curr Genet. 2001 Jun;39(4):253-63. doi: 10.1007/s002940100209.
8
Molecular cloning and characterization of a Leishmania donovani alpha-tubulin gene.杜氏利什曼原虫α-微管蛋白基因的分子克隆与特性分析
J Eukaryot Microbiol. 1995 Sep-Oct;42(5):628-32. doi: 10.1111/j.1550-7408.1995.tb05918.x.
9
The beta tubulin gene of Eimeria tenella.
Mol Biochem Parasitol. 1996 Feb-Mar;76(1-2):315-9. doi: 10.1016/0166-6851(95)02536-7.
10
Molecular characterization of four members of the alpha-tubulin gene family of the Bermuda land crab Gecarcinus lateralis.百慕大陆地蟹(Gecarcinus lateralis)α-微管蛋白基因家族四个成员的分子特征分析
J Exp Zool. 1997 Jun 1;278(2):63-77.

引用本文的文献

1
Lupinus albus gamma-tubulin: mRNA and protein accumulation during development and in response to darkness.白羽扇豆γ-微管蛋白:发育过程中及对黑暗响应时的mRNA和蛋白质积累
Planta. 2004 Jun;219(2):201-11. doi: 10.1007/s00425-004-1218-5. Epub 2004 Feb 25.
2
Gamma-tubulin in basal land plants: characterization, localization, and implication in the evolution of acentriolar microtubule organizing centers.基部陆地植物中的γ-微管蛋白:特征、定位及在无中心粒微管组织中心进化中的意义
Plant Cell. 2004 Jan;16(1):45-59. doi: 10.1105/tpc.016501. Epub 2003 Dec 5.
3
Characterization of the human homologue of the yeast spc98p and its association with gamma-tubulin.

本文引用的文献

1
A gamma-tubulin-related protein associated with the microtubule arrays of higher plants in a cell cycle-dependent manner.一种与高等植物微管阵列以细胞周期依赖性方式相关的γ-微管蛋白相关蛋白。
J Cell Sci. 1993 Apr;104 ( Pt 4):1217-28. doi: 10.1242/jcs.104.4.1217.
2
Identification of common molecular subsequences.常见分子子序列的鉴定
J Mol Biol. 1981 Mar 25;147(1):195-7. doi: 10.1016/0022-2836(81)90087-5.
3
Genomic sequencing.基因组测序
酵母spc98p的人类同源物的表征及其与γ-微管蛋白的关联。
J Cell Biol. 1998 May 4;141(3):689-701. doi: 10.1083/jcb.141.3.689.
4
Is signal transduction modulated by an interaction between heterotrimeric G-proteins and tubulin?异源三聚体G蛋白与微管蛋白之间的相互作用是否调节信号转导?
Endocrine. 1997 Oct;7(2):127-43. doi: 10.1007/BF02778134.
5
Analysis of Tub4p, a yeast gamma-tubulin-like protein: implications for microtubule-organizing center function.酵母γ-微管蛋白样蛋白Tub4p的分析:对微管组织中心功能的启示
J Cell Biol. 1996 Jul;134(2):443-54. doi: 10.1083/jcb.134.2.443.
6
A highly divergent gamma-tubulin gene is essential for cell growth and proper microtubule organization in Saccharomyces cerevisiae.一个高度分化的γ-微管蛋白基因对于酿酒酵母的细胞生长和正确的微管组织至关重要。
J Cell Biol. 1995 Dec;131(6 Pt 2):1775-88. doi: 10.1083/jcb.131.6.1775.
7
gamma-Tubulin in Arabidopsis: gene sequence, immunoblot, and immunofluorescence studies.拟南芥中的γ-微管蛋白:基因序列、免疫印迹及免疫荧光研究
Plant Cell. 1994 Feb;6(2):303-14. doi: 10.1105/tpc.6.2.303.
8
gamma-tubulin is a minus end-specific microtubule binding protein.γ-微管蛋白是一种特异性结合微管负端的蛋白质。
J Cell Biol. 1995 Oct;131(1):207-14. doi: 10.1083/jcb.131.1.207.
Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5. doi: 10.1073/pnas.81.7.1991.
4
pEMBL: a new family of single stranded plasmids.pEMBL:一类新型单链质粒。
Nucleic Acids Res. 1983 Mar 25;11(6):1645-55. doi: 10.1093/nar/11.6.1645.
5
Tubulin domains probed by limited proteolysis and subunit-specific antibodies.通过有限蛋白酶解和亚基特异性抗体探测的微管蛋白结构域
J Mol Biol. 1985 Sep 20;185(2):311-27. doi: 10.1016/0022-2836(85)90406-1.
6
Microtubule nucleating sites in higher plant cells identified by an auto-antibody against pericentriolar material.通过一种针对中心粒周围物质的自身抗体鉴定高等植物细胞中的微管成核位点。
J Cell Biol. 1985 Jul;101(1):319-24. doi: 10.1083/jcb.101.1.319.
7
An alternative approach to deoxyoligonucleotides as hybridization probes by insertion of deoxyinosine at ambiguous codon positions.通过在模糊密码子位置插入脱氧肌苷,将脱氧寡核苷酸用作杂交探针的另一种方法。
J Biol Chem. 1985 Mar 10;260(5):2605-8.
8
Anti-tubulin antibodies locate the blepharoplast during spermatogenesis in the fern Platyzoma microphyllum R.Br.: a correlated immunofluorescence and electron-microscopic study.
J Cell Sci. 1986 Mar;81:243-65. doi: 10.1242/jcs.81.1.243.
9
Progressive sequence alignment as a prerequisite to correct phylogenetic trees.渐进序列比对是构建正确系统发育树的前提条件。
J Mol Evol. 1987;25(4):351-60. doi: 10.1007/BF02603120.
10
Identification of gamma-tubulin, a new member of the tubulin superfamily encoded by mipA gene of Aspergillus nidulans.γ-微管蛋白的鉴定,它是构巢曲霉mipA基因编码的微管蛋白超家族的一个新成员。
Nature. 1989 Apr 20;338(6217):662-4. doi: 10.1038/338662a0.