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丙酸睾酮对斑鳢脑和性腺中乳酸脱氢酶及苹果酸脱氢酶的体外作用

In vitro effect of testosterone propionate on lactate dehydrogenase and malate dehydrogenase in brain and gonads of Channa punctatus.

作者信息

Gupta B L, Ansari M A, Sinha I P, Gupta S P

机构信息

Department of Zoology, Nalanda College, Bihar Sharif, India.

出版信息

Biochem Mol Biol Int. 1993 Jan;29(1):85-91.

PMID:8490571
Abstract

The activities of lactate dehydrogenase (LDH), malate dehydrogenase (MDH), and protein levels were measured in vitro incubation of tissue homogenate of brain and gonads with testosterone propionate (TP) in Channa punctatus (teleost). The activities of LDH and MDH were found to be highest in the ovarian tissues in comparison to the testis and the brain in control fish. TP (2 micrograms-4 micrograms/ml) stimulated testicular tissue with increases in LDH by 10-fold and MDH by 15-fold approximately, the ovary with significant increase in LDH and a decrease in MDH and the brain showed only an increase in MDH.

摘要

在斑鳢(硬骨鱼)中,用丙酸睾酮(TP)对脑和性腺的组织匀浆进行体外孵育,测定了乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)的活性以及蛋白质水平。与对照鱼的睾丸和脑相比,发现卵巢组织中LDH和MDH的活性最高。TP(2微克 - 4微克/毫升)刺激睾丸组织,使LDH增加约10倍,MDH增加约15倍;刺激卵巢组织,使LDH显著增加,MDH减少;而对脑而言,仅MDH增加。

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