In vitro effect of testosterone propionate on lactate dehydrogenase and malate dehydrogenase in brain and gonads of Channa punctatus.

The activities of lactate dehydrogenase (LDH), malate dehydrogenase (MDH), and protein levels were measured in vitro incubation of tissue homogenate of brain and gonads with testosterone propionate (TP) in Channa punctatus (teleost). The activities of LDH and MDH were found to be highest in the ovarian tissues in comparison to the testis and the brain in control fish. TP (2 micrograms-4 micrograms/ml) stimulated testicular tissue with increases in LDH by 10-fold and MDH by 15-fold approximately, the ovary with significant increase in LDH and a decrease in MDH and the brain showed only an increase in MDH.