Reynolds S P, Jones K P, Edwards J H, Davies B H
Asthma & Allergy Unit, Sully Hospital, South Glamorgan, UK.
Eur Respir J. 1993 Apr;6(4):467-76.
The purpose of this study was to determine the cellular events occurring 6 h after bronchial inhalation challenge, in a group of pigeon breeders. Twelve subjects agreed to undergo challenge, either with nebulized pigeon serum (Ps) (n = 10), or with saline (n = 2). The development of characteristic symptoms was used to detect a positive response in combination with monitoring tests (white blood cell count (WBC), body temperature, and spirometry). An initial bronchoalveolar lavage (BAL) was performed before inhalation challenge, and a further BAL on the previously spared right middle lobe subsegment or lingula 6 h after challenge. Paired bronchoalveolar lavage fluid (BALF) samples were analysed for urea, albumin, total and differential WBC, interleukin-1 and interleukin-2. There was a significant increase in total cells, lymphocytes, T-lymphocytes, and neutrophils; numbers.ml-1 of BALF in responders. A monitoring score reflecting severity of patient response correlated with an increase in all cell types (p < 0.05). In conclusion, responders developed both a BALF lymphocytic and neutrophilic "alveolitis" following inhalation challenge, the degree of BALF "alveolitis" correlating with the severity of patient response.
本研究的目的是确定一组信鸽饲养者在支气管吸入激发试验后6小时发生的细胞事件。12名受试者同意接受激发试验，其中10名使用雾化鸽血清（Ps），2名使用生理盐水。结合监测测试（白细胞计数（WBC）、体温和肺功能测定），通过特征性症状的出现来检测阳性反应。在吸入激发试验前进行首次支气管肺泡灌洗（BAL），并在激发试验6小时后对之前未处理的右中叶亚段或舌叶进行再次BAL。对配对的支气管肺泡灌洗液（BALF）样本进行尿素、白蛋白、总白细胞和分类白细胞、白细胞介素-1和白细胞介素-2分析。在有反应者中，BALF中的总细胞、淋巴细胞、T淋巴细胞和中性粒细胞数量显著增加；每毫升BALF中的细胞数量。反映患者反应严重程度的监测评分与所有细胞类型的增加相关（p<0.05）。总之，有反应者在吸入激发试验后出现了BALF淋巴细胞性和中性粒细胞性“肺泡炎”，BALF“肺泡炎”的程度与患者反应的严重程度相关。
