Satake N, Nagai S, Kawatani A, Kaneshima H, Tanaka S, Takeuchi M, Izumi T
2nd Dept of Internal Medicine, Kyoto University, Japan.
Eur Respir J. 1993 Apr;6(4):477-82.
In vitro studies have shown that the density of surface antigens reflects the degree of activation of T-cells. We therefore studied the density of surface antigens on T-cells from bronchoalveolar lavage fluid (BALF) and blood in patients with sarcoidosis, hypersensitivity pneumonitis (HP) and bronchiolitis obliterans with organizing pneumonia (BOOP). BALF cells were stained with anti-CD3, anti-CD4, anti-CD8 and anti-human leucocyte antigen-DR(HLA-DR) monoclonal antibodies, and were analysed by cytoflowmetry. The density was evaluated by measuring the Mean Channel fluorescence intensity of the stained cells. The results demonstrated a significant increase in the CD3 density in patients with hypersensitivity pneumonitis (108.2 +/- 20.2 MC), compared with those with pulmonary sarcoidosis (51.2 +/- 12.6), BOOP (74.5 +/- 29.3), and healthy controls (57.1 +/- 11.5). Similar results were obtained for the CD4 and CD8 density in patients with HP. Although the number of HLA-DR positive cells was increased, the density was lower in patients with sarcoidosis (57.4 +/- 11.6) and hypersensitivity pneumonitis (57.4 +/- 14.8), than in healthy controls (72.2 +/- 15.1). Comparable changes were not observed in the peripheral blood. These results suggest that T-cell activation in hypersensitivity pneumonitis may be associated with an increase in the CD3, CD4, and CD8 density on BALF T-cells.
体外研究表明，表面抗原的密度反映了T细胞的活化程度。因此，我们研究了结节病、过敏性肺炎（HP）和机化性肺炎伴闭塞性细支气管炎（BOOP）患者支气管肺泡灌洗液（BALF）和血液中T细胞表面抗原的密度。用抗CD3、抗CD4、抗CD8和抗人白细胞抗原-DR（HLA-DR）单克隆抗体对BALF细胞进行染色，并通过细胞流式仪进行分析。通过测量染色细胞的平均通道荧光强度来评估密度。结果显示，与肺结节病患者（51.2±12.6）、BOOP患者（74.5±29.3）和健康对照者（57.1±11.5）相比，过敏性肺炎患者的CD3密度显著增加（108.2±20.2 MC）。HP患者的CD4和CD8密度也得到了类似结果。尽管HLA-DR阳性细胞数量增加，但结节病患者（57.4±11.6）和过敏性肺炎患者（57.4±14.8）的密度低于健康对照者（72.2±15.1）。外周血中未观察到类似变化。这些结果表明，过敏性肺炎中的T细胞活化可能与BALF T细胞上CD3、CD4和CD8密度的增加有关。
