Finzi G, Cornaggia M, Capella C, Fiocca R, Bosi F, Solcia E, Samloff I M
Department of Human Pathology and Genetics, II Medical Faculty, University of Pavia, Varese, Italy.
Histochemistry. 1993 Mar;99(3):201-11. doi: 10.1007/BF00269138.
A specific rabbit anti-human serum was used selectively to localize the aspartic proteinase cathepsin E to follicle associated epithelium (FAE) of human and rat intestine, including jejunum, ileum, appendix, colon and rectum, as well as of human palatine, pharyngeal and lingual tonsils. Coexpression of class II histocompatibility antigen HLA-DR antigen has been observed in some of the cathepsin E-positive epithelial cells. In addition, cathepsin E has been detected in a few mononuclear cells of intestinal lymphoid structures and tonsils resembling interdigitating reticulum cells of lymph nodes. Another aspartic proteinase, cathepsin D, has been found to be poorly represented in FAE and intensely expressed by macrophages. Electron immunocytochemistry localized cathepsin E to endosomal vesicles and endoplasmic reticulum of M cells in rat and human ileum as well as of M-like cells in human palatine tonsil. The results suggest a possible role of endosomal cathepsin E in the processing of macromolecules and microorganisms transported by M cells and related epithelial cells to mucosal associated lymphoid tissue (MALT).
使用一种特异性兔抗人血清，将天冬氨酸蛋白酶组织蛋白酶E选择性地定位到人和大鼠肠道（包括空肠、回肠、阑尾、结肠和直肠）以及人腭扁桃体、咽扁桃体和舌扁桃体的滤泡相关上皮（FAE）中。在一些组织蛋白酶E阳性上皮细胞中观察到了II类组织相容性抗原HLA-DR抗原的共表达。此外，在肠道淋巴结构和扁桃体的一些单核细胞中检测到了组织蛋白酶E，这些细胞类似于淋巴结的交错突网状细胞。另一种天冬氨酸蛋白酶组织蛋白酶D在FAE中表达较少，而在巨噬细胞中强烈表达。电子免疫细胞化学将组织蛋白酶E定位到大鼠和人回肠的M细胞以及人腭扁桃体的M样细胞的内体囊泡和内质网中。结果表明，内体组织蛋白酶E可能在M细胞和相关上皮细胞向黏膜相关淋巴组织（MALT）转运的大分子和微生物的加工过程中发挥作用。
