Solcia E, Paulli M, Silini E, Fiocca R, Finzi G, Kindl S, Boveri E, Bosi F, Cornaggia M, Capella C
Dipartimento di Patologia Umana ed Ereditaria, I Facoltà di Medicina e Chirurgia, Università di Pavia ed I.R.C.C.S. Policlinico S. Matteo, Italy.
Eur J Histochem. 1993;37(1):19-26.
A specific rabbit anti-human cathepsin E serum detected this aspartic proteinase in Langerhans cells of the skin, in interdigitating reticulum cells of lymph nodes and spleen and in histiocytosis X cells, but not in macrophages. Immunoblotting of tissue extracts confirmed the presence of cathepsin E in skin and lymph nodes. Electron immunocytochemistry with the protein A gold technique localized cathepsin E in the endoplasmic reticulum and in endosomal vesicles of both interdigitating and Langerhans cells, likely involving Birbeck bodies. A role of endosomal cathepsin E in antigen processing is suggested.
一种特异性兔抗人组织蛋白酶E血清在皮肤的朗格汉斯细胞、淋巴结和脾脏的交错突网状细胞以及组织细胞增生症X细胞中检测到了这种天冬氨酸蛋白酶，但在巨噬细胞中未检测到。组织提取物的免疫印迹证实了皮肤和淋巴结中存在组织蛋白酶E。用蛋白A金技术进行的电子免疫细胞化学将组织蛋白酶E定位在交错突细胞和朗格汉斯细胞的内质网和内体小泡中，可能涉及伯贝克颗粒。提示内体组织蛋白酶E在抗原加工中起作用。
