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从大鼠皮肤中分离出降解程度最低的透明质酸。

The isolation of minimally degraded hyaluronate from rat skin.

作者信息

Mathieson J M, Pearce R H

出版信息

Biochem J. 1977 Feb 1;161(2):419-24. doi: 10.1042/bj1610419.

DOI:10.1042/bj1610419
PMID:849269
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1164519/
Abstract

Hyaluronate was isolated quantitatively from fresh rat skin by homogenization in an Edebo [J. Biochem. Microbiol. Technol. Eng. 2, (1960) 453-479] press, extraction with buffered saline, selective elution from DEAE-cellulose, and gel chromatography on Sephadex G-200. Traces of a protein contaminant remained.

摘要

通过在埃德博压榨机（《生物化学、微生物学、技术与工程杂志》2, (1960) 453 - 479）中匀浆、用缓冲盐水提取、从DEAE - 纤维素上选择性洗脱以及在葡聚糖凝胶G - 200上进行凝胶色谱法，从新鲜大鼠皮肤中定量分离出透明质酸盐。仍残留痕量蛋白质污染物。

相似文献

1
The isolation of minimally degraded hyaluronate from rat skin.从大鼠皮肤中分离出降解程度最低的透明质酸。
Biochem J. 1977 Feb 1;161(2):419-24. doi: 10.1042/bj1610419.
2
Molecular weight of hyaluronic acid from rabbit skin.兔皮中透明质酸的分子量。
J Biochem. 1977 Jan;81(1):79-91. doi: 10.1093/oxfordjournals.jbchem.a131453.
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Purification and characterization of hyaluronic acid from the horny layer of guinea pigs.豚鼠角质层中透明质酸的纯化与特性分析
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Inhibition of sulphate incorporation by chondrocytes in intact cartilage by hyaluronate from foetal cartilage.来自胎儿软骨的透明质酸盐对完整软骨中软骨细胞硫酸盐掺入的抑制作用。
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Rooster comb hyaluronate-protein, a non-covalently linked complex.鸡冠透明质酸-蛋白质，一种非共价连接的复合物。
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Migration of cranial neural crest cells in a cell-free hyaluronate-rich matrix.
Dev Biol. 1975 Jun;44(2):298-305. doi: 10.1016/0012-1606(75)90400-5.
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Hyaluronic acid in cartilage and proteoglycan aggregation.软骨中的透明质酸与蛋白聚糖聚集
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Isolation and characterization of proteohyaluronic acid from human umbilical cord.从人脐带中分离并鉴定蛋白聚糖醛酸
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引用本文的文献

1
Absence of covalently linked core protein from newly synthesized hyaluronate.新合成的透明质酸中不存在共价连接的核心蛋白。
Biochem J. 1982 Dec 1;207(3):445-57. doi: 10.1042/bj2070445.
2
Hyaluronic acid in the pulmonary secretions of patients with alveolar proteinosis.肺泡蛋白沉积症患者肺分泌物中的透明质酸
Inflammation. 1978 Jun;3(2):149-58. doi: 10.1007/BF00910736.
3
Hyaluronic acid in the pulmonary secretions of patients with asthma.哮喘患者肺分泌物中的透明质酸。
Biochem J. 1978 Aug 1;173(2):565-8. doi: 10.1042/bj1730565.

本文引用的文献

1
Preparation of hyaluronic acid from various animal sources.从各种动物来源制备透明质酸。
Ann N Y Acad Sci. 1950 May 31;52(7):971-6. doi: 10.1111/j.1749-6632.1950.tb53991.x.
2
Studies on hyaluronic acid and related substances. I. Preparation of hyaluronic acid and derivatives from human umbilical cord.透明质酸及相关物质的研究。I. 从人脐带制备透明质酸及其衍生物。
J Biol Chem. 1950 Oct;186(2):495-511.
3
THE GLYCOSAMINOGLYCAN OF THE 'GROUND SUBSTANCE' OF RAT SKIN.大鼠皮肤“基质”中的糖胺聚糖
Can J Biochem Physiol. 1963 Nov;41:2327-35.
4
THE CHEMICAL ANATOMY OF RAT SKIN.大鼠皮肤的化学剖析
Can J Biochem Physiol. 1963 Nov;41:2307-26.
5
A modified uronic acid carbazole reaction.一种改良的糖醛酸咔唑反应。
Anal Biochem. 1962 Oct;4:330-4. doi: 10.1016/0003-2697(62)90095-7.
6
Zone electrophoresis of acid mucopolysaccharides.
Can J Biochem Physiol. 1961 Nov;39:1771-82. doi: 10.1139/o61-196.
7
[Identification and estimation of glucosamine and galactosamine by paper chromatography].[通过纸色谱法鉴定和测定氨基葡萄糖和半乳糖胺]
Hoppe Seylers Z Physiol Chem. 1955 Sep 21;302(1):10-9.
8
A method for separation of chondroitin sulfate B from isomers A and C by paper electrophoresis with zinc sulphate or zinc acetate solution as electrolyte medium.
Biochim Biophys Acta. 1967 Mar 22;136(2):391-3. doi: 10.1016/0304-4165(67)90086-4.
9
Ionic polysaccharides. 3. Dilute solution properties of hyaluronic acid fractions.离子多糖。3. 透明质酸级分的稀溶液性质。
Biopolymers. 1970;9(7):799-810. doi: 10.1002/bip.1970.360090706.
10
Non-aggregated tropocollagen at physiological pH and ionic strength. A chemical and physico-chemical characterization of tropocollagen isolated from the skin of lathyritic rats.
Eur J Biochem. 1972 Feb;25(3):563-72. doi: 10.1111/j.1432-1033.1972.tb01729.x.