Brown R A, Taylor A M, McDowell J M
Institute of Orthopaedics, University of London and Middlesex School of Medicine.
Connect Tissue Res. 1990;24(2):157-68. doi: 10.3109/03008209009152431.
A number of regulators are available in cartilage to effect the local control of matrix production by chondrocytes. A cartilage slice assay has been used in this study to investigate the influence of such regulators (extracted from foetal cartilage) on intact cartilage. A net inhibition of sulphation was found, rather than stimulation as reported for extracts rich in the somatomedin-like, cartilage derived factor (CDF). Inhibition was due, to a high molecular weight component identified as hyaluronic acid (based on enzyme sensitivity, chromatographic behaviour and temperature stability). Its inhibition of sulphation in intact cartilage was more profound than that produced by commercially available umbilical cord hyaluronate. We conclude that foetal cartilage hyaluronate is a far more potent inhibitor of sulphation than hyaluronate from other sources, suppressing sulphation even in the presence of a somatomedin-like activator and in intact cartilage, which responds poorly to commercial hyaluronate.
软骨中有多种调节因子可实现对软骨细胞基质产生的局部控制。本研究采用软骨切片试验来探究此类调节因子（从胎儿软骨中提取）对完整软骨的影响。结果发现存在硫酸化的净抑制作用，而非像富含类生长调节素样软骨衍生因子（CDF）的提取物那样具有刺激作用。抑制作用归因于一种被鉴定为透明质酸的高分子量成分（基于酶敏感性、色谱行为和温度稳定性）。其对完整软骨中硫酸化的抑制作用比市售脐带透明质酸盐产生的抑制作用更为显著。我们得出结论，胎儿软骨透明质酸是比其他来源的透明质酸更强效的硫酸化抑制剂，即使在存在类生长调节素样激活剂的情况下以及在对市售透明质酸反应不佳的完整软骨中，也能抑制硫酸化。
