Ahmed J S, Wiegers P, Steuber S, Schein E, Williams R O, Dobbelaere D
Institut für Parasitologie und Tropenveterinärmedizin, FU-Berlin, Germany.
Parasitol Res. 1993;79(3):178-82. doi: 10.1007/BF00931888.
Theileria annulata and T. parva-infected lymphoblastoid cells were examined for their capacity to produce interferon (IFN). Supernatants of such cells were tested in biological assay for their antiviral activity. Only T. parva-infected cells of T-cell origin were capable of producing IFN-gamma. Supernatants of some but not all T. annulata-infected cells showed also antiviral activity, which was greatly reduced after exposure to a pH of 2. Northern-blot analysis of the cells using an IFN-gamma cDNA probe confirmed the results obtained for T. parva-infected cells in a biological assay. No IFN-gamma mRNA was detected in T. annulata-infected cells. The importance of IFN for the pathogenesis of theileriosis is discussed.
对感染环形泰勒虫和小泰勒虫的淋巴母细胞样细胞产生干扰素（IFN）的能力进行了检测。通过生物学检测法对这些细胞的上清液的抗病毒活性进行了测试。只有来源于T细胞的感染小泰勒虫的细胞能够产生γ干扰素。部分但并非所有感染环形泰勒虫的细胞的上清液也显示出抗病毒活性，在暴露于pH值为2的环境后，这种活性大幅降低。使用γ干扰素cDNA探针对细胞进行Northern印迹分析，证实了在生物学检测中针对感染小泰勒虫的细胞所获得的结果。在感染环形泰勒虫的细胞中未检测到γ干扰素mRNA。文中讨论了干扰素在泰勒虫病发病机制中的重要性。
