Kaiser W, Urbach W
Biochim Biophys Acta. 1977 Mar 11;459(3):337-46. doi: 10.1016/0005-2728(77)90035-4.
Addition of dihydroxyacetone phosphate (2.5 mM) or 3-phosphoglycerate (2.5 mM) to a suspension of isolated intact chloroplasts, which contains Pi only in low concentrations (0.2 mM) leads to a competitive inhibition of Pi uptake in the light. In consequence, the ATP/ADP ratio is strongly decreased. The rate of O2 evolution is also reduced under these conditions, but the degree of inhibition is much higher after addition of dihydroxyacetone phosphate than after addition of 3-phosphoglycerate. Therefore, besides the competitive inhibition of Pi uptake, additional effects of dihydroxyacetone phosphate and 3-phosphoglycerate on O2 evolution and CO2 fixation of isolated intact chloroplasts must occur, which are discussed.
向仅含有低浓度（0.2 mM）无机磷酸（Pi）的分离完整叶绿体悬浮液中添加磷酸二羟丙酮（2.5 mM）或3-磷酸甘油酸（2.5 mM）会导致光照下Pi摄取的竞争性抑制。结果，ATP/ADP比值大幅降低。在这些条件下，氧气释放速率也会降低，但添加磷酸二羟丙酮后的抑制程度远高于添加3-磷酸甘油酸后的抑制程度。因此，除了对Pi摄取的竞争性抑制外，磷酸二羟丙酮和3-磷酸甘油酸对分离完整叶绿体的氧气释放和二氧化碳固定必定还存在其他影响，对此进行了讨论。
