Werker P M, Green C J, Kon M, Overgoor M L, Franken R J
Department of Plastic, Reconstructive, and Hand Surgery, University Hospital, Utrecht, The Netherlands.
Ann Plast Surg. 1993 Mar;30(3):233-7; discussion 237-8. doi: 10.1097/00000637-199303000-00006.
In this study the effectiveness of a single-passage perfusion with hypertonic citrate solution on the cold ischemia tolerance in epigastric free flaps in Dark-Agouty rats was investigated. In the control group a significant decline in survival percentage was observed when the storage time was extended from 72 hours to 96 hours. A single (pressure controlled) passage perfusion with hypertonic citrate solution was able to prevent this decline.