Mullins R D, Sisken J E, Hejase H A, Sisken B F
Center for Biomedical Engineering, Lexington, KY 40506-0070.
Bioelectromagnetics. 1993;14(2):173-86. doi: 10.1002/bem.2250140209.
A system is described that is capable of producing extremely low frequency (ELF) magnetic fields for relatively short-term exposure of cultured mammalian cells. The system utilizes a ferromagnetic core to contain and direct the magnetic field of a 1,000 turn solenoidal coil and can produce a range of flux densities and induced electric fields much higher than those produced by Helmholtz coils. The system can generate magnetic fields from the microtesla (microT) range up to 0.14 T with induced electric field strengths on the order of 1.0 V/m. The induced electric field can be accurately varied by changing the sample chamber configuration without changing the exposure magnetic field. This gives the system the ability to separate the bioeffects of magnetic and induced electric fields. In the frequency range of 4-100 Hz and magnetic flux density range of 0.005-0.14 T, the maximum total harmonic distortion of the induced electric field is typically less than 1.0%. The temperature of the samples is held constant to within 0.4 degrees C by constant perfusion of warmed culture medium through the sample chamber.
描述了一种能够产生极低频（ELF）磁场，用于对培养的哺乳动物细胞进行相对短期暴露的系统。该系统利用铁磁芯来容纳和引导一个1000匝螺线管线圈的磁场，并且能够产生一系列磁通密度和感应电场，其比亥姆霍兹线圈产生的磁通密度和感应电场高得多。该系统能够产生从微特斯拉（μT）范围到0.14 T的磁场，感应电场强度约为1.0 V/m。通过改变样品腔配置可以精确改变感应电场，而不改变暴露磁场。这使该系统能够区分磁场和感应电场的生物效应。在4 - 100 Hz频率范围和0.005 - 0.14 T磁通密度范围内，感应电场的最大总谐波失真通常小于1.0%。通过将温热的培养基持续灌注通过样品腔，样品温度保持恒定在0.4℃以内。
