呼吸道合胞病毒的酶免疫测定：在鼻咽分泌物中的快速检测及对代表不同呼吸道合胞病毒亚组的分离株的评估
Enzyme immunoassay for respiratory syncytial virus: rapid detection in nasopharyngeal secretions and evaluation of isolates representing different RSV subgroups.
作者信息
Siqueira M M, Nascimento J P, Portes S A, Schuy W
机构信息
Departamento de Virologia, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
出版信息
J Clin Lab Anal. 1993;7(2):130-3. doi: 10.1002/jcla.1860070212.
Abstract
The presence of respiratory syncytial virus (RSV) was investigated by immunofluorescent antibody (IFA) technique and by an enzyme immunoassay (EIA) in 169 samples of nasopharyngeal secretions of infants and children with acute respiratory infections. Of 31 samples positive by EIA, 25 were positive by IFA. In 24 samples from a retrospective study, RSV positive by IFA and/or tissue culture isolation (TCI), 22 were also positive by EIA. The EIA was also evaluated with 111 RSV isolates in Hep2 cell cultures representing different RSV subgroups. All were positive by EIA.
摘要
采用免疫荧光抗体（IFA）技术和酶免疫测定（EIA）对169例急性呼吸道感染婴幼儿和儿童的鼻咽分泌物样本进行呼吸道合胞病毒（RSV）检测。在31例EIA检测呈阳性的样本中，25例IFA检测也呈阳性。在一项回顾性研究的24份样本中，IFA和/或组织培养分离（TCI）检测RSV呈阳性，其中22份EIA检测也呈阳性。还使用代表不同RSV亚组的111株RSV分离株在Hep2细胞培养物中对EIA进行评估。所有分离株EIA检测均呈阳性。
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