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1
Enzyme immunoassay for respiratory syncytial virus: rapid detection in nasopharyngeal secretions and evaluation of isolates representing different RSV subgroups.呼吸道合胞病毒的酶免疫测定：在鼻咽分泌物中的快速检测及对代表不同呼吸道合胞病毒亚组的分离株的评估
J Clin Lab Anal. 1993;7(2):130-3. doi: 10.1002/jcla.1860070212.
2
Rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by a commercial enzyme immunoassay.采用商用酶免疫测定法快速检测鼻咽抽吸物中的呼吸道合胞病毒。
J Clin Microbiol. 1986 Mar;23(3):485-8. doi: 10.1128/jcm.23.3.485-488.1986.
3
A rapid test for detection of respiratory syncytial virus in nasopharyngeal secretion.一种用于检测鼻咽分泌物中呼吸道合胞病毒的快速检测方法。
Eur J Clin Microbiol Infect Dis. 1990 May;9(5):356-8. doi: 10.1007/BF01973745.
4
Evaluation of clinical specimens for the presence of respiratory syncytial virus antigens using an enzyme immunoassay.使用酶免疫测定法评估临床标本中呼吸道合胞病毒抗原的存在情况。
J Med Virol. 1986 May;19(1):1-9. doi: 10.1002/jmv.1890190102.
5
Rapid detection of respiratory syncytial virus in nasopharyngeal secretions by immunofluorescence and ELISA does not justify discontinuation of virus isolation.通过免疫荧光和酶联免疫吸附测定法快速检测鼻咽分泌物中的呼吸道合胞病毒，并不足以证明可以停止病毒分离。
Microbiologica. 1989 Jul;12(3):203-13.
6
Comparison of HEp-2 cell culture and Abbott respiratory syncytial virus enzyme immunoassay.人喉表皮样癌细胞（HEp-2）细胞培养与雅培呼吸道合胞病毒酶免疫测定的比较。
J Clin Microbiol. 1987 Feb;25(2):434-6. doi: 10.1128/jcm.25.2.434-436.1987.
7
RS virus diagnosis: comparison of isolation, immunofluorescence and enzyme immunoassay.呼吸道合胞病毒诊断：病毒分离、免疫荧光法和酶免疫测定法的比较
Mem Inst Oswaldo Cruz. 1986 Apr-Jun;81(2):225-32. doi: 10.1590/s0074-02761986000200013.
8
Performance of the Kallestad Pathfinder enzyme immunoassay in the diagnosis of respiratory syncytial virus infections.卡莱斯塔德开拓者酶免疫测定法在呼吸道合胞病毒感染诊断中的性能。
Diagn Microbiol Infect Dis. 1993 May-Jun;16(4):325-9. doi: 10.1016/0732-8893(93)90084-k.
9
Comparison of the Abbott and Ortho enzyme immunoassays and cell culture for the detection of respiratory syncytial virus in nasopharyngeal specimens.雅培和奥索酶免疫测定法与细胞培养法检测鼻咽标本中呼吸道合胞病毒的比较
Diagn Microbiol Infect Dis. 1988 Apr;9(4):245-50. doi: 10.1016/0732-8893(88)90116-2.
10
Detection of respiratory syncytial virus in nasopharyngeal secretions by a biotin-avidin ELISA more sensitive than the fluorescent antibody technique.用生物素-抗生物素蛋白酶联免疫吸附测定法检测鼻咽分泌物中的呼吸道合胞病毒，其敏感性高于荧光抗体技术。
J Med Virol. 1986 Feb;18(2):113-7. doi: 10.1002/jmv.1890180203.

本文引用的文献

1
Rapid laboratory techniques for the diagnosis of viral infections.用于诊断病毒感染的快速实验室技术。
World Health Organ Tech Rep Ser. 1981;661:1-60.
2
Type-specific detection of parainfluenza viruses by enzyme-immunoassay and radioimmunoassay in nasopharyngeal specimens of patients with acute respiratory disease.通过酶免疫测定法和放射免疫测定法对急性呼吸道疾病患者鼻咽标本中的副流感病毒进行型特异性检测。
J Gen Virol. 1981 Sep;56(Pt 1):49-57. doi: 10.1099/0022-1317-56-1-49.
3
Detection of respiratory syncytial, parainfluenza type 2, and adenovirus antigens by radioimmunoassay and enzyme immunoassay on nasopharyngeal specimens from children with acute respiratory disease.采用放射免疫测定法和酶免疫测定法对患有急性呼吸道疾病儿童的鼻咽标本进行呼吸道合胞病毒、2型副流感病毒和腺病毒抗原检测。
J Clin Microbiol. 1981 Feb;13(2):258-65. doi: 10.1128/jcm.13.2.258-265.1981.
4
Epidemiology of respiratory syncytial virus infection in Washington, D.C. I. Importance of the virus in different respiratory tract disease syndromes and temporal distribution of infection.华盛顿特区呼吸道合胞病毒感染的流行病学。一、该病毒在不同呼吸道疾病综合征中的重要性及感染的时间分布。
Am J Epidemiol. 1973 Sep;98(3):216-25. doi: 10.1093/oxfordjournals.aje.a121550.
5
Comparison of washed nasopharyngeal cells and whole nasal secretions for detection of respiratory syncytial virus antigens by enzyme-linked immunosorbent assay.通过酶联免疫吸附测定法比较洗涤后的鼻咽细胞和全鼻分泌物用于检测呼吸道合胞病毒抗原
J Clin Microbiol. 1986 Feb;23(2):383-4. doi: 10.1128/jcm.23.2.383-384.1986.
6
Detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay, indirect immunofluorescence, and virus isolation: a comparative study.通过酶联免疫吸附测定、间接免疫荧光和病毒分离法检测鼻咽分泌物中的呼吸道合胞病毒：一项比较研究。
J Med Virol. 1986 Jun;19(2):123-34. doi: 10.1002/jmv.1890190204.
7
Detection of respiratory syncytial virus in nasopharyngeal secretions by a biotin-avidin ELISA more sensitive than the fluorescent antibody technique.用生物素-抗生物素蛋白酶联免疫吸附测定法检测鼻咽分泌物中的呼吸道合胞病毒，其敏感性高于荧光抗体技术。
J Med Virol. 1986 Feb;18(2):113-7. doi: 10.1002/jmv.1890180203.
8
The selection and performance of monoclonal and polyclonal anti-respiratory syncytial virus (RS) antibodies in capture ELISAs for antigen detection.用于抗原检测的捕获ELISA中，单克隆和多克隆抗呼吸道合胞病毒（RS）抗体的选择与性能。
J Virol Methods. 1987 Sep;17(3-4):247-61. doi: 10.1016/0166-0934(87)90135-2.
9
Antigenic relatedness between glycoproteins of human respiratory syncytial virus subgroups A and B: evaluation of the contributions of F and G glycoproteins to immunity.人呼吸道合胞病毒A、B亚组糖蛋白之间的抗原相关性：F和G糖蛋白对免疫作用的评估
J Virol. 1987 Oct;61(10):3163-6. doi: 10.1128/JVI.61.10.3163-3166.1987.
10
RS virus diagnosis: comparison of isolation, immunofluorescence and enzyme immunoassay.呼吸道合胞病毒诊断：病毒分离、免疫荧光法和酶免疫测定法的比较
Mem Inst Oswaldo Cruz. 1986 Apr-Jun;81(2):225-32. doi: 10.1590/s0074-02761986000200013.

呼吸道合胞病毒的酶免疫测定：在鼻咽分泌物中的快速检测及对代表不同呼吸道合胞病毒亚组的分离株的评估

Enzyme immunoassay for respiratory syncytial virus: rapid detection in nasopharyngeal secretions and evaluation of isolates representing different RSV subgroups.

作者信息

Siqueira M M, Nascimento J P, Portes S A, Schuy W

机构信息

Departamento de Virologia, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.

出版信息

J Clin Lab Anal. 1993;7(2):130-3. doi: 10.1002/jcla.1860070212.

DOI:10.1002/jcla.1860070212
PMID:8505698
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7166710/
Abstract

The presence of respiratory syncytial virus (RSV) was investigated by immunofluorescent antibody (IFA) technique and by an enzyme immunoassay (EIA) in 169 samples of nasopharyngeal secretions of infants and children with acute respiratory infections. Of 31 samples positive by EIA, 25 were positive by IFA. In 24 samples from a retrospective study, RSV positive by IFA and/or tissue culture isolation (TCI), 22 were also positive by EIA. The EIA was also evaluated with 111 RSV isolates in Hep2 cell cultures representing different RSV subgroups. All were positive by EIA.

摘要

采用免疫荧光抗体（IFA）技术和酶免疫测定（EIA）对169例急性呼吸道感染婴幼儿和儿童的鼻咽分泌物样本进行呼吸道合胞病毒（RSV）检测。在31例EIA检测呈阳性的样本中，25例IFA检测也呈阳性。在一项回顾性研究的24份样本中，IFA和/或组织培养分离（TCI）检测RSV呈阳性，其中22份EIA检测也呈阳性。还使用代表不同RSV亚组的111株RSV分离株在Hep2细胞培养物中对EIA进行评估。所有分离株EIA检测均呈阳性。