Wang C, Lee G S, Leung A, Surrey E S, Chan S Y
Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, California 90048.
Fertil Steril. 1993 Jun;59(6):1221-7.
To determine the relationships between human sperm hyperactivation (HA) and acrosome reaction with fertilization of human oocytes in vitro.
Prospective analysis of infertile patients undergoing IVF.
Tertiary center for infertility and assisted reproductive technology.
Fifty-two couples who underwent IVF or GIFT were studied. The male partners had normal semen parameters on routine analyses.
None.
Sperm HA in seminal fluid and after 1 and 6 hours incubation in capacitation media; sperm acrosome reaction after 0, 6, and 24 hours' incubation in capacitation media; and fertilization rate of human oocytes.
The percent spermatozoa with transitional HA after 6 hours of incubation correlated significantly with the fertilization rate of human oocytes. Multivariate discriminant analyses selected six sperm HA and acrosome reaction variables of predictive value in classifying semen samples that achieved fertilization rates above and below 70% threshold. Combination of the selected sperm HA and acrosome reaction variables in seminal fluid and after different times of incubation in capacitation medium classified the samples achieving good or poor fertilization rate with > 80% accuracy.
Assessment of sperm HA and acrosome reaction may be of prognostic value in prediction of human oocyte fertilization rates.
确定人类精子超激活（HA）和顶体反应与人类卵母细胞体外受精之间的关系。
对接受体外受精的不育患者进行前瞻性分析。
不孕症与辅助生殖技术三级中心。
研究了52对接受体外受精或配子输卵管内移植（GIFT）的夫妇。男性伴侣的常规精液分析参数正常。
无。
精液中的精子HA以及在获能培养基中孵育1小时和6小时后的精子HA；在获能培养基中孵育0小时、6小时和24小时后的精子顶体反应；以及人类卵母细胞的受精率。
孵育6小时后具有过渡性HA的精子百分比与人类卵母细胞的受精率显著相关。多变量判别分析选择了六个精子HA和顶体反应变量，这些变量在对受精率高于和低于70%阈值的精液样本进行分类时具有预测价值。精液中以及在获能培养基中不同孵育时间后的选定精子HA和顶体反应变量的组合，对受精率高或低的样本进行分类，准确率>80%。
评估精子HA和顶体反应可能对预测人类卵母细胞受精率具有预后价值。
