红大麻哈鱼（Oncorhynchus nerka）斯坦尼小体中鲷鱼钙蛋白mRNA的原位检测与分布

In situ detection and distribution of stanniocalcin mRNA in the corpuscles of Stannius of sockeye salmon, Oncorhynchus nerka.

作者信息

Sterba T, Wagner G F, Schroedter I C, Friesen H G

机构信息

Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.

出版信息

Mol Cell Endocrinol. 1993 Jan;90(2):179-85. doi: 10.1016/0303-7207(93)90150-i.

DOI:10.1016/0303-7207(93)90150-i

PMID:8495799

Abstract

Stanniocalcin (STC) gene expression was examined in the corpuscles of Stannius of sockeye salmon by in situ hybridization. Generally, the STC gene was not expressed uniformly in all cells of the corpuscles of Stannius. However, in cases where gene expression was uniform throughout a gland, it appeared that virtually all cells contained STC mRNA, thereby supporting the 'one cell type' hypothesis for the corpuscles of Stannius. Correlative immunocytochemistry on adjacent tissue sections indicated that cellular levels of stored hormone paralleled the distribution of STC mRNA. Stanniocalcin mRNA was undetectable in numerous other salmon tissues, thereby further supporting the notion that the STC gene is expressed exclusively in the corpuscles of Stannius.

摘要

通过原位杂交技术检测了红大马哈鱼斯坦尼小体中钙调蛋白（STC）基因的表达。一般来说，STC基因在斯坦尼小体的所有细胞中并非均匀表达。然而，在整个腺体中基因表达均匀的情况下，似乎几乎所有细胞都含有STC mRNA，从而支持了斯坦尼小体的“单一细胞类型”假说。对相邻组织切片进行的相关免疫细胞化学表明，储存激素的细胞水平与STC mRNA的分布平行。在许多其他鲑鱼组织中未检测到钙调蛋白mRNA，从而进一步支持了STC基因仅在斯坦尼小体中表达的观点。