Topper E K, Colditz I G, Windon R G
CSIRO Division of Animal Health, Armidale, N.S.W., Australia.
Vet Immunol Immunopathol. 1992 Apr;32(1-2):65-75. doi: 10.1016/0165-2427(92)90069-3.
A study was undertaken on the capacity of platelet-activating factor (PAF) to induce eosinophil accumulation in the mammary glands of non-lactating sheep. Platelet-activating factor induced dose-dependent accumulation of eosinophils in mammary exudates 24 h after infusion. Infection, by intraruminal injection of 20,000 infective Trichostrongylus colubriformis larvae, did not modify the responsiveness of outbred sheep to intramammary infusion of PAF. Mature ewes from high and low responder lines of a flock of sheep, selected on the basis of their responses to vaccination and experimental challenge with T. colubriformis as lambs, did not differ in the magnitude of the eosinophil responses to doses of PAF from 5 x 10(-13) to 5 x 10(-7) mol per gland. Intramammary infusion of an extract from third stage larvae of Haemonchus contortus elicited inflammatory exudates containing five- to ten-fold more eosinophils than that elicited by the highest dose of PAF tested. The experiments indicate that the eosinophil chemotactic agonist PAF can induce tissue eosinophilia in sheep and thus may play a role in directing the accumulation of eosinophils in tissues during disease states such as gastrointestinal parasitism.
开展了一项关于血小板活化因子（PAF）诱导非泌乳期绵羊乳腺中嗜酸性粒细胞积聚能力的研究。输注血小板活化因子24小时后，可诱导乳腺渗出液中嗜酸性粒细胞呈剂量依赖性积聚。通过瘤胃内注射20,000条感染性蛇形毛圆线虫幼虫进行感染，并未改变杂种绵羊对乳腺内输注PAF的反应性。根据作为羔羊时对疫苗接种和蛇形毛圆线虫实验性攻击的反应，从一群绵羊中挑选出高反应性和低反应性品系的成年母羊，它们对每腺体5×10⁻¹³至5×10⁻⁷摩尔剂量的PAF的嗜酸性粒细胞反应强度并无差异。乳腺内输注捻转血矛线虫三期幼虫的提取物所引发的炎性渗出液中嗜酸性粒细胞数量比所测试的最高剂量PAF引发的渗出液中嗜酸性粒细胞数量多五至十倍。这些实验表明，嗜酸性粒细胞趋化激动剂PAF可诱导绵羊组织嗜酸性粒细胞增多，因此在诸如胃肠寄生虫病等疾病状态下，可能在引导嗜酸性粒细胞在组织中积聚方面发挥作用。
