Prokopová A, Kéry V, Stancíková M, Grimová J, Capek P, Sandula J, Orviský E
Research Institute of Rheumatic Diseases, Piestany, Czechoslovakia.
J Rheumatol. 1993 Apr;20(4):673-7.
Methyl-alpha-D-mannopyranoside, mannooligosaccharides obtained by acetolysis of yeast mannan, and pure mannans isolated from the cell walls of pathogenic (Candida albicans) and nonpathogenic (Saccharomyces cerevisiae) yeasts were used for treatment of rat adjuvant arthritis. The arthritis was induced by the application of Freund's complete adjuvant into the tail region of rats. The mannose substances were injected into the arthritic rats intraperitonealy at different time intervals. Levels of serum albumin, changes in hindpaws swelling and radiographs were measured in infected rats as variables of the inflammation and destructive arthritic changes. While mannan from C. albicans inhibited both the inflammation and destructive arthritic changes, mannan from S. cerevisiae showed less effect. However, acetolysate of S. cerevisiae mannan as well as simple methyl-alpha-D-mannopyranoside inhibited both inflammation and destructive arthritic changes to a similar degree as mannan isolated from C. albicans. The effect, which is not dose dependent indicates its possible immunoregulatory mechanism. This is the first time a therapeutic effect of simple carbohydrates on rat adjuvant arthritis has been described.
α-D-甲基甘露糖苷、通过酵母甘露聚糖乙酰解获得的甘露寡糖，以及从致病性酵母（白色念珠菌）和非致病性酵母（酿酒酵母）细胞壁中分离出的纯甘露聚糖，被用于治疗大鼠佐剂性关节炎。关节炎是通过将弗氏完全佐剂注射到大鼠尾部区域诱导产生的。甘露糖物质在不同时间间隔腹腔注射到患有关节炎的大鼠体内。在受感染的大鼠中测量血清白蛋白水平、后爪肿胀变化和X光片，作为炎症和破坏性关节炎变化的变量。虽然白色念珠菌的甘露聚糖抑制了炎症和破坏性关节炎变化，但酿酒酵母的甘露聚糖效果较差。然而，酿酒酵母甘露聚糖的乙酰解产物以及简单的α-D-甲基甘露糖苷对炎症和破坏性关节炎变化的抑制程度与从白色念珠菌中分离出的甘露聚糖相似。这种不依赖剂量的效应表明其可能的免疫调节机制。这是首次描述简单碳水化合物对大鼠佐剂性关节炎的治疗作用。
