Vyas M N, Vyas N K, Meikle P J, Sinnott B, Pinto B M, Bundle D R, Quiocho F A
Department of Biochemistry, Baylor College of Medicine, Houston, TX 77030.
J Mol Biol. 1993 May 5;231(1):133-6. doi: 10.1006/jmbi.1993.1262.
The Fab of a monoclonal anti-carbohydrate antibody, SYA/J6 (IgG3, kappa, murine), raised against the O-polysaccharide antigen of the cell surface lipopolysaccharide of variant Y Shigella flexneri, a Gram negative bacterium, has been crystallized in the unliganded form and in complex with tri- and pentasaccharide antigens. The three crystal forms belong to the tetragonal space group P4(3)2(1)2, or P4(1)2(1)2, with very similar unit cell dimensions and an asymmetric unit that contains one molecule of about 50,000 Daltons, and a fourth crystal form belongs to monoclinic space group P2(1) that contains four molecules of Fab in an asymmetric unit. Whereas diffractions of these crystals on an area detector-rotating anode system extend to only about 3.5 A resolution, those measured using an imaging plate and synchrotron radiation at the Photon Factory facility extend to 2.5 A.
一种抗碳水化合物单克隆抗体SYA/J6（IgG3，κ链，鼠源）的Fab片段已分别以未结合配体形式以及与三糖和五糖抗原形成复合物的形式结晶。该抗体是针对革兰氏阴性细菌福氏志贺氏菌Y变种细胞表面脂多糖的O-多糖抗原产生的。这三种晶体形式属于四方晶系空间群P4(3)2(1)2或P4(1)2(1)2，其晶胞尺寸非常相似，不对称单元包含一个约50,000道尔顿的分子，第四种晶体形式属于单斜晶系空间群P2(1)，其不对称单元包含四个Fab分子。这些晶体在面积探测器 - 旋转阳极系统上的衍射仅延伸到约3.5埃分辨率，而使用成像板和光子工厂设施的同步辐射测量的衍射则延伸到2.5埃。
