Rijke R P, Plaisier H M, de Ruiter H, Galjaard H
Gastroenterology. 1977 May;72(5 Pt 1):896-901.
The effect of experimental bypass on the intestinal epithelium of the rat was investigated from 2 to 360 days after creating a Thiry-Vella fistula. Within 7 to 14 days the number of cells per villus column and, to a lesser degree, the number of cells per crypt column decreased and subsequently these values remained constant up to 1 year. Radioautography did not show any changes in the percentage of labelled cells per crypt column, after incorporation of [3H]thymidine. In addition, the relative size of the proliferative cell compartment in the crypt, the number of crypts per unit length of small intestine and the crypt-villus ratio remained unchanged. Scintillation counting of isolated crypts from the Thiry-Vella fistulas showed that the number of cells per crypt was markedly reduced. Scanning electron microscopy revealed that the three-dimensional size of the villi was also reduced. The activities of nonspecific esterases and neutral alpha-glucosidase remained unchanged in crypt cells and were above control values in villus cells. The development of these biochemical characteristics of the villus cells seems to be independent of the luminal contents. It was concluded that deprivation of food passage and gastric, pancreatic, and liver secretions from a small intestinal segment primarily causes a reduction in the number of cells per crypt, which leads to a lower cell production. In turn, this is responsible for the reduction of the functional villus cell compartment.
在制作了蒂里 - 韦拉瘘管后2至360天，研究了实验性肠旁路对大鼠肠上皮的影响。在7至14天内，每个绒毛柱的细胞数量以及每个隐窝柱的细胞数量在较小程度上减少，随后这些值在长达1年的时间内保持恒定。在掺入[3H]胸苷后，放射自显影未显示每个隐窝柱标记细胞百分比有任何变化。此外，隐窝中增殖细胞区室的相对大小、每单位小肠长度的隐窝数量以及隐窝 - 绒毛比率均保持不变。对来自蒂里 - 韦拉瘘管的分离隐窝进行闪烁计数表明，每个隐窝的细胞数量明显减少。扫描电子显微镜显示绒毛的三维尺寸也减小。隐窝细胞中非特异性酯酶和中性α - 葡糖苷酶的活性保持不变，而绒毛细胞中的活性高于对照值。绒毛细胞这些生化特征的发展似乎与肠腔内容物无关。得出的结论是，小肠段食物通过以及胃、胰腺和肝脏分泌物的缺失主要导致每个隐窝细胞数量减少，这导致细胞产生减少。反过来，这又导致功能性绒毛细胞区室减少。
