Keren D F, Holt P S, Collins H H, Gemski P, Formal S B
Infect Immun. 1980 Jun;28(3):950-6. doi: 10.1128/iai.28.3.950-956.1980.
Several variables inherent in chronically isolated ileal (Thiry-Vella) loops in rabbits were studied for their effect on the local immune response of the intestine to live, locally invasive bacteria (Shigella X16). A much more vigorous local immunoglobulin A response to Shigella X16 was elicited when rabbits were immunized in their Thiry-Vella loops shortly after surgical creation of the loop than if a week were allowed to pass before they were immunized. Three major differences existed in Thiry-Vella loops on the day after surgery and a week later: (i) their microbial flora, (ii) nonspecific acute inflammation due to the surgery itself, and (iii) the histological appearance of the intestine. On day 1 after surgical creation of the Thiry-Vella loop, there were few bacteria in the loop, and the histology was that of normal small bowel except for mild acute inflammation due to the surgery. By day 6 after surgery, all loops contained large numbers of Pseudomonas aeruginosa and other aerobes, an atrophy of intestinal epithelium occurred, and the acute inflammation due to surgical trauma had subsided. By artificially colonizing Thiry-Vella loops with 10(8) or 10(10) live P. aeruginosa on the day of surgery, we found that the presence of these bacteria alone did not greatly diminish local immune responses to live Shigella. Furthermore, when the acute inflammation due to surgical trauma was recreated in loops 6 days old, no enhancement of the immune response was seen as compared to nontraumatized 6-day-old Thiry-Vella loops. The difference between immunization soon after surgery and a week later related to changes that occur in the loop itself with increased isolation. Finally, multiple immunizations of Thiry-Vella loops resulted in a more vigorous local immunoglobulin A response than a single immunization. These studies demonstrated that Thiry-Vella loop models can be useful in studying the kinetics of local immune responses by the intestine only if careful attention is paid to key variables inherent in the Thiry-Vella loop models themselves.
研究了兔慢性分离回肠（Thiry-Vella）袢中固有的几个变量对肠道针对活的、局部侵袭性细菌（志贺氏菌X16）的局部免疫反应的影响。与在手术创建袢一周后才进行免疫相比，在手术创建Thiry-Vella袢后不久就在其中对兔子进行免疫，能引发对志贺氏菌X16更强烈的局部免疫球蛋白A反应。手术当天和一周后的Thiry-Vella袢存在三个主要差异：（i）其微生物菌群，（ii）手术本身引起的非特异性急性炎症，以及（iii）肠道的组织学外观。在手术创建Thiry-Vella袢后的第1天，袢内细菌很少，组织学表现为正常小肠，只是因手术有轻度急性炎症。到术后第6天，所有袢内都含有大量铜绿假单胞菌和其他需氧菌，肠上皮出现萎缩，手术创伤引起的急性炎症已消退。通过在手术当天用10⁸或10¹⁰个活的铜绿假单胞菌人工定殖Thiry-Vella袢，我们发现仅这些细菌的存在并不会大幅降低对活志贺氏菌的局部免疫反应。此外，当在6天大的袢中重现手术创伤引起的急性炎症时，与未受创伤的6天大的Thiry-Vella袢相比，未观察到免疫反应增强。术后不久免疫和一周后免疫的差异与随着隔离时间增加袢本身发生的变化有关。最后，对Thiry-Vella袢进行多次免疫比单次免疫能引发更强烈的局部免疫球蛋白A反应。这些研究表明，只有仔细关注Thiry-Vella袢模型本身固有的关键变量，Thiry-Vella袢模型才有助于研究肠道局部免疫反应的动力学。
