Sprague S M, Krieger N S, Bushinsky D A
Nephrology Program, University of Chicago, Illinois 60637.
Am J Physiol. 1993 May;264(5 Pt 2):F882-90. doi: 10.1152/ajprenal.1993.264.5.F882.
Cells isolated from neonatal mouse calvariae can be induced to form mineralized nodules after exposure to ascorbic acid and beta-glycerophosphate. To determine whether aluminum inhibits nodule formation and subsequent mineralization, cells isolated from neonatal mouse calvariae were induced to form nodules and incubated with increasing concentrations of aluminum (10(-7) to 10(-5) M). Compared with control and 10(-7) M aluminum-supplemented cultures, the number of nodules formed and the number of nodules calcified were reduced in cells incubated with 10(-6) and 10(-5) M aluminum. The cumulative net calcium uptake into the nodules and their final calcium content were also decreased with 10(-6) and 10(-5) M aluminum. After 10 and 18 days of incubation, aluminum did not affect DNA synthesis or release of alkaline phosphatase but significantly inhibited collagen production. Thus aluminum induced a dose-dependent inhibition of nodule formation and calcification that may be related to its inhibition of collagen production.
从新生小鼠颅骨分离出的细胞在暴露于抗坏血酸和β-甘油磷酸后可被诱导形成矿化结节。为了确定铝是否抑制结节形成及随后的矿化，从新生小鼠颅骨分离出的细胞被诱导形成结节，并与浓度不断增加的铝（10⁻⁷至10⁻⁵M）一起孵育。与对照和补充10⁻⁷M铝的培养物相比，在与10⁻⁶和10⁻⁵M铝一起孵育的细胞中，形成的结节数量和钙化的结节数量减少。用10⁻⁶和10⁻⁵M铝处理后，结节中钙的累积净摄取量及其最终钙含量也降低。孵育10天和18天后，铝不影响DNA合成或碱性磷酸酶的释放，但显著抑制胶原蛋白的产生。因此，铝诱导了结节形成和钙化的剂量依赖性抑制，这可能与其对胶原蛋白产生的抑制有关。
