Garrick R A, Ryan U S, Bower V, Cua W O, Chinard F P
Fordham University, College at Lincoln Center, Division of Science and Mathematics, New York, NY 10023-7475.
Biochim Biophys Acta. 1993 May 14;1148(1):108-16. doi: 10.1016/0005-2736(93)90166-w.
The diffusional permeability coefficients, PD, for tritiated water (3HHO) 14C-antipyrine (AP) and 14C-iodoantipyrine (IAP) in isolated calf pulmonary artery endothelial cells and dog erythrocytes are measured with the linear diffusion technique at 11.5, 15, 20 and 37 degrees C. The PD values for both cell populations follow the sequence 3HHO > IAP > AP at each of the temperatures. PD for water is higher in the erythrocyte compared to the endothelial cells. The differences in PD for AP and IAP in the erythrocytes and endothelial cells are not dramatic and are similar to the differences seen in comparing permeation of the same solute through bilayers of different composition. A comparison of the values of PD calculated for the endothelial cells with those for isolated capillaries and the structured endothelium in whole lungs validates the use of the isolated cells as models for the endothelial cells in situ. Incubation of the endothelial cells with cis-vaccenic acid or cholesterol produces a reduction in PD for water and antipyrine. These data are analyzed in terms of Stokesian and non-Stokesian diffusion. The interpretation which best accommodates the data is that the phospholipid area of the membrane, rather than the hydrocarbon core, provides the greatest resistance to permeation for these solutes.
采用线性扩散技术，在11.5、15、20和37摄氏度下，测量了氚标记水（3HHO）、14C-安替比林（AP）和14C-碘安替比林（IAP）在分离的小牛肺动脉内皮细胞和犬红细胞中的扩散渗透系数PD。在每个温度下，两种细胞群体的PD值均遵循3HHO>IAP>AP的顺序。与内皮细胞相比，红细胞中水的PD更高。红细胞和内皮细胞中AP和IAP的PD差异不显著，与比较同一溶质通过不同组成双层的渗透差异相似。将内皮细胞计算的PD值与分离的毛细血管和全肺结构内皮的PD值进行比较，证实了使用分离细胞作为原位内皮细胞模型的有效性。用顺式- vaccenic酸或胆固醇孵育内皮细胞会导致水和安替比林的PD降低。这些数据根据斯托克斯扩散和非斯托克斯扩散进行了分析。最符合数据的解释是，膜的磷脂区域而非烃核，对这些溶质的渗透提供了最大阻力。
