Garrick R A, Chinard F P
Am J Physiol. 1982 Nov;243(5):C285-92. doi: 10.1152/ajpcell.1982.243.5.C285.
Membrane permeability coefficients (P0) of rabbit lung cells consisting primarily of alveolar epithelial and endothelial cells and of alveolar macrophages from dog lungs were determined for tritiated water, n-[14C]alcohols, and [14C]antipyrine over the temperature range 10 to 37 degrees C with the series-parallel pathway model. In the mixed cell preparation both the diffusional permeability to water (755 X 10(-5) cm.s-1 at 37 degrees C) and the response to temperature change (apparent activation energy, Ea, 10 kcal.mol-1) are greater than the corresponding values in the macrophages (110 X 10(-5) cm.s-1 and 4.8 kcal.mol-1, respectively). The permeability coefficients for the small alcohols (C1-C3) are similar and considerably higher than for water in both cellular preparations. The values of the permeability coefficients and the temperature dependence for antipyrine and the larger alcohols in the mixed lung cells differ from the values obtained in the macrophages. Comparison of our results with those obtained in erythrocytes and Novikoff hepatoma cells demonstrates the differences in water permeability in each cell preparation and the similarity in permeation for the more lipophilic solutes in the cell preparations. These differences may be important in the comparison of results obtained in isolated cellular systems and in intact tissues and organs.
采用串并联途径模型，在10至37摄氏度的温度范围内，测定了主要由肺泡上皮细胞、内皮细胞组成的兔肺细胞以及犬肺肺泡巨噬细胞对氚水、n-[¹⁴C]醇类和[¹⁴C]安替比林的膜渗透系数（P₀）。在混合细胞制剂中，对水的扩散渗透性（37摄氏度时为755×10⁻⁵厘米·秒⁻¹）和对温度变化的响应（表观活化能，Ea，10千卡·摩尔⁻¹）均大于巨噬细胞中的相应值（分别为110×10⁻⁵厘米·秒⁻¹和4.8千卡·摩尔⁻¹）。在两种细胞制剂中，小分子醇类（C1 - C3）的渗透系数相似，且远高于水的渗透系数。混合肺细胞中安替比林和较大醇类的渗透系数值及其温度依赖性与巨噬细胞中的值不同。将我们的结果与红细胞和诺维科夫肝癌细胞中的结果进行比较，表明每种细胞制剂中水渗透性的差异以及细胞制剂中亲脂性更强的溶质渗透的相似性。这些差异在比较分离细胞系统以及完整组织和器官中获得的结果时可能很重要。
