Mearow K M, Kril Y, Diamond J
Division of Anatomy, McMaster University Medical Centre, Hamilton, Ontario, Canada.
Neuroreport. 1993 Apr;4(4):351-4. doi: 10.1097/00001756-199304000-00002.
Using a coupled reverse transcription and competitive polymerase chain reaction protocol, we have measured Nerve Growth Factor (NGF) mRNA expression in adult rat skin following its denervation. By 2-4 days, levels of NGF mRNA were increased approximately 5-10 fold over levels in innervated skin, remaining increased for up to at least 2 weeks. In situ hybridization carried out on skin samples revealed that the NGF message was expressed not only in the distal nerve pathways but also in non-nerve associated cells, such as dermal fibroblasts, and in the basal epidermal layer. Denervated skin evokes a NGF-dependent collateral sprouting from neighbouring intact sensory axons. The new findings indicate that an increased NGF production is associated with the increased availability of NGF in such skin, and that cutaneous nerves have a role in regulating this NGF production.
通过使用逆转录和竞争性聚合酶链反应相结合的方法，我们测量了成年大鼠皮肤去神经支配后神经生长因子（NGF）mRNA的表达。在2 - 4天内，NGF mRNA水平比有神经支配的皮肤中的水平增加了约5 - 10倍，并至少持续增加2周。对皮肤样本进行的原位杂交显示，NGF信息不仅在远端神经通路中表达，也在非神经相关细胞如真皮成纤维细胞和基底表皮层中表达。去神经支配的皮肤会引起邻近完整感觉轴突的NGF依赖性侧支芽生。新发现表明，NGF产量的增加与这种皮肤中NGF可用性的增加有关，并且皮肤神经在调节这种NGF产生中发挥作用。
