Filipek A, Puzianowska M, Cieślak B, Kuźnicki J
Nencki Institute of Experimental Biology, Warsaw, Poland.
Neuroreport. 1993 Apr;4(4):383-6. doi: 10.1097/00001756-199304000-00010.
The aim of this work was to confirm the presence of calcylin in brain and to identify calcyclin positive cells. Calcyclin was identified in brain soluble proteins that were bound to phenyl-sepharose in a calcium dependent fashion. Specific antibodies against calcylin were found to stain subsets of brain neurones such as neurones in Ammons horn of hippocampus, granule cells in cerebellum, and neurones in brain stem. Glial cells which contain large amounts of S-100 beta protein were calcyclin negative. These results indicate that calcyclin is present in neurones, but not in glial cells.
这项工作的目的是确认脑钙调素在大脑中的存在，并鉴定脑钙调素阳性细胞。在以钙依赖方式与苯基琼脂糖结合的脑可溶性蛋白中鉴定出脑钙调素。发现针对脑钙调素的特异性抗体可对脑神经元的亚群进行染色，例如海马齿状回中的神经元、小脑中的颗粒细胞以及脑干中的神经元。含有大量S-100β蛋白的神经胶质细胞为脑钙调素阴性。这些结果表明脑钙调素存在于神经元中，而不存在于神经胶质细胞中。
