Nencki Institute of Experimental Biology, 3 Pasteur Street, 02-093, Warsaw, Poland.
Neurochem Res. 2013 Sep;38(9):1941-8. doi: 10.1007/s11064-013-1100-6. Epub 2013 Jul 2.
In the present work we examined expression and localization of the S100A6 protein in rat brain in a model of epilepsy induced by Status Epilepticus evoked by amygdala stimulation. We demonstrate, through the use of the reverse transcriptase-polymerase chain reaction technique, that mRNA level of S100A6 was increased in cortex while, as found by immunoblotting, the level of the S100A6 protein was significantly higher in the cortex and in the CA1 area of the hippocampus at day 14 after stimulation. Immunohistochemical studies performed on rat brain slices indicated that S100A6 immunoreactivity was elevated in GFAP-positive astrocytes in the hippocampus and cortex starting from day 1, and further increased at day 4 and 14 after stimulation. Interestingly, in a subpopulation of astrocytes, up-regulation of S100A6 was associated with an increased level of β-catenin, a protein involved in regulation of S100A6 expression. Altogether, our data show a widespread and prolonged up-regulation of S100A6 in the epileptic brain and indicate that an increase in S100A6 immunoreactivity is related to astrogliosis.
在本研究中，我们通过逆转录聚合酶链反应技术检测到 S100A6 mRNA 在杏仁核刺激诱导癫痫发作模型大鼠脑中的表达和定位。我们发现，S100A6 蛋白水平在刺激后 14 天明显升高，而免疫印迹分析显示 S100A6 蛋白水平在皮质和海马 CA1 区显著升高。对大鼠脑切片进行的免疫组织化学研究表明，S100A6 免疫反应性在海马和皮质中的 GFAP 阳性星形胶质细胞中从第 1 天开始升高，并在刺激后第 4 天和第 14 天进一步升高。有趣的是，在星形胶质细胞的一个亚群中，S100A6 的上调与 β-连环蛋白水平的升高有关，β-连环蛋白是一种参与 S100A6 表达调控的蛋白质。总之，我们的数据表明 S100A6 在癫痫脑中广泛且持续上调，并表明 S100A6 免疫反应性的增加与星形胶质细胞增生有关。
