一种用于人类卵裂球X和Y同时原位杂交的快速高效方法。
A fast and efficient method for simultaneous X and Y in situ hybridization of human blastomeres.
作者信息
Munné S, Weier H U, Stein J, Grifo J, Cohen J
机构信息
Gamete and Embryo Research Laboratory, New York Hospital-Cornell University Medical Center, New York 10021.
出版信息
J Assist Reprod Genet. 1993 Jan;10(1):82-90. doi: 10.1007/BF01204446.
PURPOSE
To use a 6-hr fluorescence in situ hybridization (FISH) procedure involving fluorochrome-labeled probes to determine the gender of blastomeres from arrested biopsied human embryos.
RESULTS
Simultaneous detection of X and Y chromosomes was performed on 68 blastomeres with this technique. The FISH efficiency for gender determination was 95.5% (65/68). In addition, rehybridization with chromosome 8-specific probes was performed to determine the ploidy of blastomeres with more than two sex chromosomes.
CONCLUSION
This technique offers an alternative to polymerase chain reaction for the preimplantation diagnosis of X-linked diseases and can also be used for ploidy assessment.
目的
采用一种6小时的荧光原位杂交（FISH）程序，该程序涉及荧光染料标记的探针，以确定取自停止发育的活检人类胚胎的卵裂球的性别。
结果
使用该技术对68个卵裂球同时进行了X和Y染色体检测。性别确定的FISH效率为95.5%（65/68）。此外，用8号染色体特异性探针进行再杂交，以确定具有两条以上性染色体的卵裂球的倍性。
结论
该技术为X连锁疾病的植入前诊断提供了一种替代聚合酶链反应的方法，也可用于倍性评估。
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