Dryden M S, Talsania H, Rodgers J, Bayston R, Phillips I
Department of Microbiology, United Medical School, St. Thomas' Hospital, London, UK.
Eur J Clin Microbiol Infect Dis. 1993 Feb;12(2):87-92. doi: 10.1007/BF01967580.
Sera and dialysis effluent from 20 patients on continuous ambulatory peritoneal dialysis (CAPD) with coagulase-negative staphylococcal (CNS) peritonitis were examined by immunoblotting for antibody activity against CNS. Immunoblotting was highly sensitive and demonstrated significantly greater antibody activity in serum and dialysate of infected patients compared with that of uninfected CAPD patients or healthy volunteers. Fourteen of 20 infected CAPD patients had strong antibody activity (> 7 bands); one patient had equivocal activity. Five patients had weak antibody activity, two of whom suffered from recurrent peritonitis with distinguishable CNS strains despite a satisfactory CAPD technique. One patient with a poor CAPD technique had strong antibody activity, but suffered from recurrent peritonitis. Examination of sequential sera suggested that seroconversion occurred soon after insertion of the Tenckhoff catheter, possibly in the absence of clinical infection. Antibody activity against a 25 kDa staphylococcal protein was significantly associated with peritonitis.
对20例持续性非卧床腹膜透析（CAPD）合并凝固酶阴性葡萄球菌（CNS）腹膜炎患者的血清和透析液进行免疫印迹检测，以检测其抗CNS的抗体活性。免疫印迹法高度敏感，与未感染的CAPD患者或健康志愿者相比，感染患者血清和透析液中的抗体活性明显更高。20例感染的CAPD患者中有14例具有强抗体活性（>7条带）；1例患者活性不明确。5例患者抗体活性较弱，其中2例尽管CAPD技术令人满意，但仍患有复发性腹膜炎，且CNS菌株可区分。1例CAPD技术不佳的患者具有强抗体活性，但患有复发性腹膜炎。对连续血清的检测表明，在插入Tenckhoff导管后不久可能在无临床感染的情况下发生了血清转化。抗25 kDa葡萄球菌蛋白的抗体活性与腹膜炎显著相关。
