Hassan M M, Ata M, Ramzy R M, el-Gendi A E, Hegab M H, Gabr N S, el-Chourbagy A F, Mostafa A A, Naguib A M
Department of Parasitology, Faculty of Medicine, University, Egypt.
J Egypt Soc Parasitol. 1996 Dec;26(3):687-96.
Four groups of patients were selected: 16 patients with clinical evidence of obstructive filarial lymphangiopathy without microfilaraemia; 12 patients with clinical evidence of obstructive filarial lymphangiopathy with microfilaraemia and 9 patients with microfilaraemia. Two control groups were also included. Blood films, sera and hydrocele fluid samples were collected from all subjects. Polyclonal antibody against Dirofilaria immitis worm homogenate was prepared, fractionated and conjugated with HRP. Both polyclonal antibody and monoclonal antibody (AD12) were used in a sandwich ELISA. Using polyclonal antibody, both microfilaraemic groups (groups 2 and 3) had a significantly higher mean O.D. readings than that of control groups (P < 0.05), whereas, the mean O.D. readings of patients with symptomatic amicrofilaraemia had no significant difference than control groups. Symptomatic microfilaraemic group had the highest percentage of antigen positivity 7/12 (58.3%) among all groups while symptomatic amicrofilaraemic group had the least antigen positivity 2/16 (12.5%). Patients presented with elephantiasis only or with hydrocele had no antigen positive levels 0/12 (0%) in their serum or hydrocele fluid samples. On the other hand, 2 out of 4 cases represented with hydrocele (50%) had positive antigen levels in their hydrocele fluid samples. Using monoclonal antibody, all groups had a highly significant higher mean optical density readings than control groups. Asymptomatic microfilaraemic group had the highest percentage of antigen positivity 8/9 (88.9%), followed by symptomatic microfilaraemic 8/12 (66.7%), then symptomatic amicrofilaraemic group 4/16 (25%). The same 2 patients (amicrofilaraemia) and 7 (microfilaraemia) represented with hydrocele had positive antigen levels in their hydrocele fluid samples.
16例有阻塞性丝虫性淋巴管病临床证据但无微丝蚴血症的患者；12例有阻塞性丝虫性淋巴管病临床证据且有微丝蚴血症的患者以及9例有微丝蚴血症的患者。还纳入了两个对照组。从所有受试者采集血涂片、血清和鞘膜积液样本。制备了抗犬恶丝虫虫体匀浆的多克隆抗体，进行分级分离并与辣根过氧化物酶（HRP）偶联。多克隆抗体和单克隆抗体（AD12）均用于夹心酶联免疫吸附测定（ELISA）。使用多克隆抗体时，两个有微丝蚴血症的组（第2组和第3组）的平均光密度读数均显著高于对照组（P<0.05），而有症状的无微丝蚴血症患者的平均光密度读数与对照组无显著差异。有症状的微丝蚴血症组在所有组中抗原阳性率最高，为7/12（58.3%），而有症状的无微丝蚴血症组抗原阳性率最低，为2/16（12.5%）。仅表现为象皮肿或鞘膜积液的患者，其血清或鞘膜积液样本中无抗原阳性水平，为0/12（0%）。另一方面，4例表现为鞘膜积液的病例中有2例（50%）其鞘膜积液样本中抗原水平呈阳性。使用单克隆抗体时，所有组的平均光密度读数均显著高于对照组。无症状微丝蚴血症组抗原阳性率最高，为8/9（88.9%），其次是有症状微丝蚴血症组8/12（66.7%），然后是有症状无微丝蚴血症组4/16（25%）。同样，2例表现为鞘膜积液的无微丝蚴血症患者和7例有微丝蚴血症患者的鞘膜积液样本中抗原水平呈阳性。
