Michoud E, Carpentier P, Franco A, Intaglietta M
Laboratoire d'Hémodynamique Périphérique et de Microcirculation, Université Joseph Fourier, Centre Hospitalier Régional Universitaire de Grenoble, France.
Int J Microcirc Clin Exp. 1993 Apr;12(2):157-71.
The Lambert-Beer's law of the absorption of the light by blood in a vessel is used to model the light attenuation by a blood vessel that is transilluminated. Two models are used for an automatic vessel diameter determination for intravital microscopy. Some requirements for the photometric system have to be met in order to reduce errors due to light scattering. In these conditions, a videodensitometric pattern of the cross-section of the vessel can be fitted by the different models in order to obtain the diameter of the vessel. The first model proposed uses a uniformly distributed red blood cell column. A non-linear estimation of the diameter is done with the Levenberg-Marquardt method in 2 sec, using a regular PC386 microcomputer. The second one takes in account the presence of a plasma layer and computes the diameter of the red blood cell column and the diameter of the vessel in one minute. These models can be used for pharmacological studies or for a better understanding of the formation of a transilluminated intravital image. They can also be used for angiographic images.
血管中血液对光的吸收的朗伯 - 比尔定律用于对透照血管的光衰减进行建模。两种模型用于通过活体显微镜自动测定血管直径。为了减少由于光散射引起的误差，必须满足光度系统的一些要求。在这些条件下，血管横截面的视频密度测定模式可以通过不同模型进行拟合，以获得血管直径。提出的第一个模型使用均匀分布的红细胞柱。使用常规的PC386微型计算机，采用列文伯格 - 马夸尔特方法在2秒内对直径进行非线性估计。第二个模型考虑了血浆层的存在，并在一分钟内计算红细胞柱的直径和血管的直径。这些模型可用于药理学研究或更好地理解透照活体图像的形成。它们也可用于血管造影图像。
